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In vivo protein trapping produces a functional expression codex of the vertebrate proteome.
Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
31827pGBT-R16
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31828pGBT-RP2-1
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47117pT3TS-CreCan be used to in vitro transcribe Cre recombinase.
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47118pCR4-*mRFPCan be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
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