Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.
Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 36201 | pLifeAct-mTurquoise2 | In vivo visualization of actin cytoskeleton (can be used for colocalization studies) |
| 36202 | pmTurquoise2-Tubulin | In vivo visualization of microtubules (can be used for colocalization studies) |
| 36203 | pmTurquoise2-Peroxi | In vivo visualization of the peroxisome (can be used for colocalization studies) |
| 36204 | pmTurquoise2-ER | In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies) |
| 36205 | pmTurquoise2-Golgi | In vivo visualization of golgi apparatus (can be used for colocalization studies) |
| 36206 | pmTurquoise2-NES | In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies) |
| 36207 | pmTurquoise2-H2A | In vivo visualization of the nucleus (can be used for colocalization studies) |
| 36208 | pmTurquoise2-Mito | In vivo visualization of the mitochondria (can be used for colocalization studies) |
| 36209 | pPalmitoyl-mTurquoise2 | In vivo visualization of the membrane (can be used for colocalization studies) |
| 54842 | mTurquoise2-C1 | Localization: C1 Cloning Vector, Excitation: 434, Emission: 474 |
| 54843 | mTurquoise2-N1 | Localization: N1 Cloning Vector, Excitation: 434, Emission: 474 |
| 60493 | pmVenus(L68V)-mTurquoise2 | Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V) |
| 60494 | pmTurquoise2-T2A-Venus(L68V) | Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET. |
| 60560 | pmTurquoise2-C1 | Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins |
| 60561 | pmTurquoise2-N1 | Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins |