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Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.
Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
36201pLifeAct-mTurquoise2In vivo visualization of actin cytoskeleton (can be used for colocalization studies)
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36202pmTurquoise2-TubulinIn vivo visualization of microtubules (can be used for colocalization studies)
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36203pmTurquoise2-PeroxiIn vivo visualization of the peroxisome (can be used for colocalization studies)
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36204pmTurquoise2-ERIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)
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36205pmTurquoise2-GolgiIn vivo visualization of golgi apparatus (can be used for colocalization studies)
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36206pmTurquoise2-NESIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)
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36207pmTurquoise2-H2AIn vivo visualization of the nucleus (can be used for colocalization studies)
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36208pmTurquoise2-MitoIn vivo visualization of the mitochondria (can be used for colocalization studies)
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36209pPalmitoyl-mTurquoise2In vivo visualization of the membrane (can be used for colocalization studies)
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54842mTurquoise2-C1Localization: C1 Cloning Vector, Excitation: 434, Emission: 474
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54843mTurquoise2-N1Localization: N1 Cloning Vector, Excitation: 434, Emission: 474
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60493pmVenus(L68V)-mTurquoise2Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)
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60494pmTurquoise2-T2A-Venus(L68V)Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.
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60560pmTurquoise2-C1Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins
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60561pmTurquoise2-N1Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins
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