Cho SW, Kim S, Kim JM, Kim JS
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507. PubMed Article
A human codon-optimized Streptococcus pyogenes Cas9 nuclease under the control of the CMV promoter. The resulting Cas9 enzyme, when expressed with a gRNA, was shown to efficiently cleave two genomic sites and induce InDels with frequencies of up to 33% in a human cell line.
Plasmids from Article