Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein.
Dammeyer T, Timmis KN, Tinnefeld P
Microb Cell Fact. 2013 May 20;12(1):49.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
45936pTD-NStrepHisuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag
Loading...
45937pTD-NTwinStrep_Smuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag
Loading...
45938pTD-NTwinStrep_Kmuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag
Loading...
45939pTD-CTwinStrepuse to create C-terminal Twin-Strep-tag fusion proteins
Loading...
45940pTDpelB-NTwinStrepuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
Loading...
45941pTDpelB-CTwinStrepuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence
Loading...
45942pTD-C_eYFPTwinStrepexpresses E.coli codon optimized EYFP-Twin-Strep fusion protein
Loading...
45943pTD-C_sfYFPTwinStrepexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein
Loading...
45944pTDpelB-C_sfYFPTwinStrepexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
Loading...