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Broad host range vectors for expression of proteins with (Twin-) Strep-tag, His-tag and engineered, export optimized yellow fluorescent protein.
Dammeyer T, Timmis KN, Tinnefeld P
Microb Cell Fact. 2013 May 20;12(1):49.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
45936pTD-NStrepHisuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag
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45937pTD-NTwinStrep_Smuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag
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45938pTD-NTwinStrep_Kmuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag
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45939pTD-CTwinStrepuse to create C-terminal Twin-Strep-tag fusion proteins
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45940pTDpelB-NTwinStrepuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
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45941pTDpelB-CTwinStrepuse to create N-terminal PelB and C-terminal Twin-Strep-tagged fusion proteins for periplasmic translocation via PelB signal sequence
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45942pTD-C_eYFPTwinStrepexpresses E.coli codon optimized EYFP-Twin-Strep fusion protein
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45943pTD-C_sfYFPTwinStrepexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein
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45944pTDpelB-C_sfYFPTwinStrepexpresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence
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