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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L, Church GM
Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
47314Cas9m2Cas9 D10A+H840A
47315Cas9m3Cas9 D10A+D839A+H840A
47316Cas9m4Cas9 D10A+D839A+H840A+N863A
47317Cas9m2-VP64Cas9m2 Activator
47318Cas9m3-VP64Cas9m3 Activator
47319Cas9m4-VP64Cas9m4 Activator
47320reporter-gT1TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)
47321reporter-gT2TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)