Lacritin rescues stressed epithelia via rapid forkhead box O3 (FOXO3)-associated autophagy that restores metabolism.
Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW
J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 10.1074/jbc.M112.436584. Epub 2013 May 2. PubMed Article
Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler MF 4th, Lee JK, Laurie GW
J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 10.1074/jbc.M112.436584. Epub 2013 May 2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 48718 | pTYB1-pLAC-N45 | Bacterial expression of Lacritin protein with 45 AA removed from N terminus |
| 48720 | pTYB1-pLAC-N71 | Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus |
| 48721 | pTYB1-pLAC-V69S | Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine |
| 48722 | pTYB1-pLAC-I73S | Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine |
| 48723 | pTYB1-pLAC-I98S | Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine |
| 48725 | pTYB-pLAC-L108S | Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine |
| 48727 | pTYB-pLAC-F112S | Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine |
| 48728 | pTYB-pLAC-I68S/I73S | Bacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to Serine |
| 48730 | pTYB-pLAC-L108S/L109S/F112S | Bacterial expression of Lacritin Protein with Mutation at proteins 108 and 109 Leucine to Serine and 112 from phenylalanine to serine |