Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.

Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
50716	pCAG-EGxxFP	5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
50717	pCAG-EGxxFP-Cetn1	Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.
50718	pX330-Cetn1/1	pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.