Derivation of novel human ground state naive pluripotent stem cells.
Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. PubMed Article
Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52379 | OCT4-GFP-2A-PURO transgenic reporter | BAC recombineering generated construct, used as a reporter for OCT4 expression |
| 52380 | delta PE OCT4-GFP-2A-PURO transgenic reporter | BAC recombineering generated construct, used as a reporter for OCT4 expression |
| 52381 | delta DE OCT4-GFP-2A-PURO transgenic reporter | BAC recombineering generated construct, used as a reporter for OCT4 expression |
| 52382 | mouse deltaPE Oct4-GFP transgenic reporter | BAC recombineering generated construct, used as a reporter for mouse Oct4 expression |
| 52412 | FOR human OCT4 TALEN | TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939) |
| 52413 | REV human OCT4 TALEN | TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939) |
| 52414 | pGL3-human OCT4 DE-SV40-Luc | Luciferase reporter plasmid for DE OCT4 enhancer activity |
| 52415 | pGL3-human OCT4 PE-SV40-Luc | Luciferase reporter plasmid for PE OCT4 enhancer activity |
| 52419 | PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO | Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette. |
| 60527 | mouse Oct4-GFP GOF18 transgenic reporter | BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements) |