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Derivation of novel human ground state naive pluripotent stem cells.
Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH
Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 2013 Oct 30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52379OCT4-GFP-2A-PURO transgenic reporterBAC recombineering generated construct, used as a reporter for OCT4 expression
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52380delta PE OCT4-GFP-2A-PURO transgenic reporter BAC recombineering generated construct, used as a reporter for OCT4 expression
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52381delta DE OCT4-GFP-2A-PURO transgenic reporterBAC recombineering generated construct, used as a reporter for OCT4 expression
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52382mouse deltaPE Oct4-GFP transgenic reporterBAC recombineering generated construct, used as a reporter for mouse Oct4 expression
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52412FOR human OCT4 TALENTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
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52413REV human OCT4 TALENTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
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52414pGL3-human OCT4 DE-SV40-LucLuciferase reporter plasmid for DE OCT4 enhancer activity
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52415pGL3-human OCT4 PE-SV40-LucLuciferase reporter plasmid for PE OCT4 enhancer activity
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52419PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PUROConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.
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60527mouse Oct4-GFP GOF18 transgenic reporterBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)
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