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An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility.
Rao MV, Chu PH, Hahn KM, Zaidel-Bar R
Cytoskeleton (Hoboken). 2013 Jul;70(7):394-407. doi: 10.1002/cm.21115. Epub 2013 May 24.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
45583GFP-CA-mDia1
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53095PA-DAD-wtExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.
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53096PA-DAD-darkExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformation
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