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Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system.
Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI
Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68311pENTR5'-attP (pCM254)Gateway middle entry vector for full-length attP site for phiC31-mediated transgenesis.
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68313pDestattB (pCM268)Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish
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68339pDestattB_ubi:EGFP (pCM272)Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.
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68341pDestattB/CY (pCM327)Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.
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