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Degradation of the Deubiquitinating Enzyme USP33 is Mediated by p97 and the Ubiquitin Ligase HERC2.
Chan NC, den Besten W, Sweredoski MJ, Hess S, Deshaies RJ, Chan DC
J Biol Chem. 2014 May 22. pii: jbc.M114.569392. PubMed Article

Plasmids from Article

ID Plasmid Purpose
55613pcDNA5 FRT/TO SF-HERC2 (ShB-R)Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
55614pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells
55784pcDNA SF-HERC2 F1Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)
55785pcDNA SF-HERC2 F2Expresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)
55786pcDNA SF-HERC2 F3Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)
55787pcDNA SF-HERC2 F4Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)
55788pcDNA SF-HERC2 F5Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)
55789pcDNA SF-HERC2 F6Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Antibodies from Article