Reversible protein inactivation by optogenetic trapping in cells.
Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. PubMed Article
Lee S, Park H, Kyung T, Kim NY, Kim S, Kim J, Heo WD
Nat Methods. 2014 Jun;11(6):633-6. doi: 10.1038/nmeth.2940. Epub 2014 May 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58366 | pCMV-CIB1-mCerulean-MP | For use in light-induced protein inactivation through the interaction with CRY2 |
| 58367 | pCMV-CIB1-mRFP1-MP | For use in light-induced protein inactivation through the interaction with CRY2 |
| 58368 | pCMV-CRY2-mCherry | Mamlian codon optimized CRY2 |
| 58369 | pCMV-CRY2(D387A)-mCherry | Light insensitive CRY2 mutant |
| 58370 | pCMV-SNAP-CRY2-VHH(GFP) | For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP |