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Recruitment of Mad1 to metaphase kinetochores is sufficient to reactivate the mitotic checkpoint.
Ballister ER, Riegman M, Lampson MA
J Cell Biol. 2014 Mar 17;204(6):901-8. doi: 10.1083/jcb.201311113. PubMed Article

Plasmids from Article

ID Plasmid Purpose
58280pERB109constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB
58281pERB111constitutive miRempty_Mis12-GFP-FKBP3; inducible mCh-FRB
58282pERB122const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB
58283pERB127const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)
58284pERB130const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1
58285pERB160const. Mis12-FKBP3; ind. FRB-mCH-Mad1
58286pERB161const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)
58288pERB170const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-584
58289pERB171const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718
58290pERB172const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-718-AAA
58291pERB174const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^549-718

Antibodies from Article