MicroRNA-based single-gene circuits buffer protein synthesis rates against perturbations.
Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. Epub 2014 Jan 30. PubMed Article
Strovas TJ, Rosenberg AB, Kuypers BE, Muscat RA, Seelig G
ACS Synth Biol. 2014 May 16;3(5):324-31. doi: 10.1021/sb4001867. Epub 2014 Jan 30. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59877 | sgFFL | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene. |
| 59891 | sgFFL_d3 | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated. |
| 59892 | sgFFL_d23 | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated |
| 59893 | sgFFL_3x_siRNA | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites |
| 59894 | sgFFL_7x_Bulge | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sites |
| 59895 | OpenLoopControl | synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Does not contain the target containing Vamp3 3′UTR |