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MIR144 and MIR451 regulate human erythropoiesis via RAB14.
Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI
Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60934miR-144 Trapto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter
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61487miR-451 Trapto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoter
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61488miR-144-451 Trapto knockdown endogenous miR144 and miR451 levels
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61489RAB14 3'UTR WTluciferase reporter containing RAB14 3' UTR
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61490RAB14 3'UTR D1luciferase reporter containing RAB14 3' UTR with the first predicted MiR144 binding site deleted
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61491RAB14 3'UTR D2luciferase reporter containing RAB14 3' UTR with the second predicted MiR144 binding site deleted
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61492RAB14 3'UTR D1D2luciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deleted
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61493RAB14 WTto overexpress wild type RAB14. Vector includes GFP driven by hUbC promoter
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61494RAB14 Q70Lto overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter
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61495RAB14 S25Nto overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter
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61496RAB14 shRNAto knockdown endogenous RAB14 expression levels
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