Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Fauser F, Schiml S, Puchta H
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. PubMed Article
Fauser F, Schiml S, Puchta H
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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61432 | pEn-Chimera | Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone |
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61433 | pDe-CAS9 | Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence |
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61476 | pChimera | Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone |
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61477 | pDe-CAS9-D10A | based on pDe-CAS9, encodes Cas9 D10A nickase variant |
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61478 | pCAS9-TPC | Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning |
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