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Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
Fauser F, Schiml S, Puchta H
Plant J. 2014 Jul;79(2):348-59. doi: 10.1111/tpj.12554. Epub 2014 Jun 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
61432pEn-ChimeraGateway-Entry vector containing AtU6-26 promoter and sgRNA backbone
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61433pDe-CAS9Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence
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61476pChimeraVector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone
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61477pDe-CAS9-D10Abased on pDe-CAS9, encodes Cas9 D10A nickase variant
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61478pCAS9-TPCBinary expression vector with A.th. codon-optimized Cas9 for conventional cloning
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Antibodies from Article