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The duplicated alpha7 subunits assemble and form functional nicotinic receptors with the full-length alpha7.
Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA
J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 10.1074/jbc.M114.582858. Epub 2014 Jul 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62276pcDNA3.1-CHRNA7mammalian expression of human Alpha7 (CHRNA7)
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62284pcDNA3.1-CHRFAM7Amammalian expression of human duplicated Alpha7 (CHRFAM7A)
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62515pcDNA3.1-CHRFAM7ADelta2bpmammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)
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62629pcDNA3.1-CHRNA7-mGFPmammalian expression of human Alpha7 (CHRNA7) with mGFP fused into M3-M4 loop
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62630pcDNA3.1-CHRNA7-YFPmammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop
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62632pcDNA3.1-CHRNA7-mCherrymammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop
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62633pcDNA3.1-CHRFAM7A-mGFPmammalian expression of human duplicated Alpha7 (CHRFAM7A) with mGFP fused into M3-M4 loop
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62634pcDNA3.1-CHRFAM7A-YFPmammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop
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62635pcDNA3.1-CHRFAM7A-mCherrymammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop
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62636pcDNA3.1-CHRFAM7ADelta2bp-mGFPmammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mGFP fused into M3-M4 loop
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62637pcDNA3.1-CHRFAM7ADelta2bp-YFPmammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop
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62638pcDNA3.1-CHRFAM7ADelta2bp-mCherrymammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop
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Antibodies from Article