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Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry.
Meyer AJ, Ellefson JW, Ellington AD
ACS Synth Biol. 2014 Oct 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
63622pQE-WT-CTGA-R13-AKSRVCTGA-R13-AKSRV RNAP mutant
63623pQE-WT-T3-R5-RRVHT3-R5-RRVH RNAP mutant
63624pQE-WT-KIF-R5-KIKRKIF-R5-KIKR RNAP mutant
63625pQE-WT-N4-R5-YRNRVN4-R5-YRNRV RNAP mutant
63626pQE-WT-CGG-R12-KIRCGG-R12-KIR RNAP mutant
63627pQE-WT-CTGA-R13-AKSIRVCTGA-R13-AKSIRV RNAP mutant
63628pQE-WT-T3-R5-RVT3-R5-RV RNAP mutant
63629pQE-WT-KIF-R5-IRHK1F-R5-IRH RNAP mutant
63630pQE-WT-N4-R5-RNRVN4-R5-RNRV RNAP mutant
63668pQE-WT-CGG-R12-KIRVCGG-R12-KIRV RNAP mutant

Antibodies from Article