Combinatorial mutagenesis of the voltage-sensing domain enables the optical resolution of action potentials firing at 60 Hz by a genetically encoded fluorescent sensor of membrane potential.
Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
J Neurosci. 2015 Jan 7;35(1):372-85. doi: 10.1523/JNEUROSCI.3008-14.2015. PubMed Article
Piao HH, Rajakumar D, Kang BE, Kim EH, Baker BJ
J Neurosci. 2015 Jan 7;35(1):372-85. doi: 10.1523/JNEUROSCI.3008-14.2015. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63713 | CC1 | Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain |
| 63714 | CC2 | Voltage sensing domain of Ciona with the D136A/A154D/E183N/R223Q mutations fused to super ecliptic pHlorin A227D |
| 63715 | CC3 | Voltage sensing domain of Ciona with the G122A/D164A/D186A/R217Q/R229I mutations fused to super ecliptic pHlorin A227D |
| 63716 | CC4 | Voltage sensing domain of Ciona with the D129S/R168A/D186S/R229I mutations fused to super ecliptic pHlorin A227D |
| 63717 | CC5 | Voltage sensing domain of Ciona with the D129N/D164N/G187A/V220T mutations fused to super ecliptic pHlorin A227D |
| 63718 | CC6 | Voltage sensing domain of Ciona with the D136S/R168H/T193P/V220R mutations fused to super ecliptic pHlorin A227D |
| 63719 | Triple mutant | Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D |
| 63720 | Bongwoori | Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D |