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A versatile modular vector system for rapid combinatorial mammalian genetics.
Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, Bode-Lesniewska B, Wild PJ, Frew IJ
J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62084pMuLE ENTR MCS L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62085pMuLE ENTR MCS L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62086pMuLE ENTR MCS R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62087pMuLE ENTR MCS L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62088pMuLE ENTR MCS L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62089pMuLE ENTR MCS L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning
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62090pMuLE ENTR CMV L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62091pMuLE ENTR CMV L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62092pMuLE ENTR CMV L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62093pMuLE ENTR CMV R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62094pMuLE ENTR CMV loxP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning
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62095pMuLE ENTR SV40 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62096pMuLE ENTR SV40 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62097pMuLE ENTR SFFV L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62098pMuLE ENTR SFFV L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62099pMuLE ENTR CMV/TO L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62100pMuLE ENTR CMV/TO R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning
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62101pMuLE ENTR H1 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62102pMuLE ENTR H1 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62103pMuLE ENTR H1 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62104pMuLE ENTR H1 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62105pMuLE ENTR H1 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62106pMuLE ENTR 7SK L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62107pMuLE ENTR 7SK L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62108pMuLE ENTR 7SK L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62109pMuLE ENTR 7SK R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62110pMuLE ENTR 7SK L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62111pMuLE ENTR U6 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62112pMuLE ENTR U6 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter for shRNA expression. Compatible with MultiSite Gateway cloning
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62113pMuLE ENTR U6-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62114pMuLE ENTR U6-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62115pMuLE ENTR U6-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62116pMuLE ENTR U6-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62117pMuLE ENTR U6-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62118pMuLE ENTR 7SK-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62119pMuLE ENTR 7SK-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62120pMuLE ENTR 7SK-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62121pMuLE ENTR 7SK-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62122pMuLE ENTR CMV/TO-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62123pMuLE ENTR CMV/TO-miR-30 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62124pMuLE ENTR CMV/TO-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62125pMuLE ENTR CMV/TO-miR-30 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62126pMuLE ENTR CMV/TO-miR-30 L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a tet-inducible CMV promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62127pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
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62128pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
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62129pMuLE ENTR U6 stuffer sgRNA scaffold L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
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62130pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
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62131pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
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62132pMuLE ENTR CMV-hCas9 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
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62133pMuLE ENTR SV40-hCas9 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
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62134pMuLE ENTR SV40-hCas9 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
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62135pMuLE ENTR U6 n.s shRNA L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
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62136pMuLE ENTR U6 n.s shRNA L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
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62137pMuLE ENTR U6 n.s shRNA R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
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62138pMuLE ENTR U6 n.s shRNA L5-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
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62139pMuLE ENTR U6 n.s shRNA L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning
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62140pMuLE ENTR CMV eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62141pMuLE ENTR CMV eGFP R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62142pMuLE ENTR CMV eGFP L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62143pMuLE ENTR CMV eGFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62144pMuLE ENTR SV40 eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62145pMuLE ENTR SV40 eGFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning
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62146pMuLE ENTR CMV mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
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62147pMuLE ENTR CMV mCherry R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
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62148pMuLE ENTR CMV mCherry L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and mCherry module. Compatible with MultiSite Gateway cloning
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62149pMuLE ENTR SV40 mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning
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62150pMuLE ENTR SV40 mCherry L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloning
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62151pMuLE ENTR CMV tdTomato R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
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62152pMuLE ENTR CMV tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
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62153pMuLE ENTR SV40 tdTomato L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning
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62154pMuLE ENTR SV40 tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and tdTomato module. Compatible with MultiSite Gateway cloning
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62155pMuLE ENTR SFFV tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SFFV promoter and tdTomato module. Compatible with MultiSite Gateway cloning
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62156pMuLE ENTR CMV iRFP L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
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62157pMuLE ENTR CMV iRFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
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62158pMuLE ENTR CMV iRFP R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
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62159pMuLE ENTR CMV iRFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning
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62160pMuLE ENTR SV40 iRFP L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning
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62161pMuLE ENTR CMV PuroR L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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62162pMuLE ENTR CMV PuroR R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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62163pMuLE ENTR CMV PuroR L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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62164pMuLE ENTR SV40 PuroR L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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62165pMuLE ENTR SV40 PuroR L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning
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62166pMuLE ENTR CMV LacZ L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning
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62167pMuLE ENTR SV40 LacZ L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and LacZ module. Compatible with MultiSite Gateway cloning
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62168pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
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62169pMuLE ENTR CMV Luc2 (FF-Luciferase) R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
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62170pMuLE ENTR SV40 Luc2 (FF-Luciferase) L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
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62171pMuLE ENTR SV40 Luc2 (FF-Luciferase) L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning
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62172pMuLE ENTR CMV CreERT2 L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
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62173pMuLE ENTR CMV CreERT2 R4-R3MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
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62174pMuLE ENTR SV40 CreERT2 L3-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and CreERT2 module. Compatible with MultiSite Gateway cloning
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62175pMuLE Lenti Dest eGFPMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
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62176pMuLE Lenti Dest iRFPMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
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62177pMuLE Lenti Dest IFP1.4MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
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62178pMuLE Lenti Dest NeoMuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
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62179pMuLE Lenti Dest Luc2MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
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62180pMuLE ENTR 7SK-miR-30 L1-L4MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62181pMuLE ENTR H1-miR-30 L1-R5MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
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62183pMuLE ENTR eGFP L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning
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62184pMuLE ENTR mCherry L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing mCherry. Compatible with MultiSite Gateway cloning
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62185pMuLE ENTR tdTomato L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning
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62186pMuLE ENTR CMV Renilla Luciferase L5-L2MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning
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