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Yeast GPCR-sensor Toolkit
(Kit # 1000000157 )

Depositing Lab:   Tom Ellis

The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Öling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. PubMed PMID: 30955892.

Description

The Ellis Lab Yeast GPCR-sensor Toolkit consists of 42 parts for building highly-tunable GPCR-based biosensors in yeast. The parts are formatted for use with, and require, MoClo-YTK (Kit #1000000061) from the Dueber Lab (Lee et al., 2015), and are designed to work with the engineered yeast strain yWS677 (soon available from ATCC).

Note from the depositor: Part pWS932 contains a 50/50 ratio of C/A at position 662, which could lead to the substitution of a serine to a tyrosine (S235Y). We recommend sequencing assembled plasmids using this part to confirm the presence of the correct base pair at this position.

Ordering Note: If you would like to order both the Yeast GPCR-sensor Toolkit and MoClo-YTK (Kit #1000000061), or have previously ordered MoClo-YTK (Kit #1000000061) and would now like to order the Yeast GPCR-sensor Toolkit, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Ellis Yeast GPCR-sensor Toolkit
  • Art by Sigrid Knemeyer, SciStories LLC, scistories.com.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Yeast GPCR-sensor Toolkit was a gift from Tom Ellis (Addgene kit #1000000157).”

For your Reference section:

Engineering a Model Cell for Rational Tuning of GPCR Signaling. Shaw WM, Yamauchi H, Mead J, Gowers GF, Bell DJ, Öling D, Larsson N, Wigglesworth M, Ladds G, Ellis T. Cell. 2019 Apr 18;177(3):782-796.e27. doi: 10.1016/j.cell.2019.02.023. PubMed PMID: 30955892.

Yeast GPCR-sensor Toolkit - #1000000157

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Chloramphenicol

Inventory

Well Plasmid Resistance
A / 1 pWS060
Chloramphenicol
A / 2 pWS053
Chloramphenicol
A / 3 pWS2247
Chloramphenicol
A / 4 pWS1078
Chloramphenicol
A / 5 pWS480
Chloramphenicol
A / 6 pWS481
Chloramphenicol
A / 7 pWS482
Chloramphenicol
A / 8 pWS483
Chloramphenicol
A / 9 pWS484
Chloramphenicol
A / 10 pWS485
Chloramphenicol
A / 11 pWS494
Chloramphenicol
A / 12 pWS491
Chloramphenicol
B / 1 pWS487
Chloramphenicol
B / 2 pWS493
Chloramphenicol
B / 3 pRC246
Chloramphenicol
B / 4 pWS486
Chloramphenicol
B / 5 pWS498
Chloramphenicol
B / 6 pWS496
Chloramphenicol
B / 7 pWS497
Chloramphenicol
B / 8 pWS495
Chloramphenicol
B / 9 pWS067
Chloramphenicol
B / 10 pWS626
Chloramphenicol
B / 11 pWS625
Chloramphenicol
B / 12 pWS1806
Chloramphenicol
C / 1 pWS2119
Chloramphenicol
C / 2 pWS1037
Chloramphenicol
C / 3 pWS936
Chloramphenicol
C / 4 pWS939
Chloramphenicol
C / 5 pWS926
Chloramphenicol
C / 6 pWS931
Chloramphenicol
C / 7 pWS930
Chloramphenicol
C / 8 pWS932
Chloramphenicol
C / 9 pWS033
Chloramphenicol
C / 10 pRC063
Chloramphenicol
C / 11 pWS1837
Chloramphenicol
C / 12 pWS788
Chloramphenicol
D / 1 pWS081
Chloramphenicol
D / 2 pWS509
Chloramphenicol
D / 3 pWS510
Chloramphenicol
D / 4 pWS511
Chloramphenicol
D / 5 pWS627
Chloramphenicol
D / 6 pWS1859
Chloramphenicol
Data calculated @ 2019-11-19

Kit Plate Map - #1000000157

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