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MoChlo: Modular Cloning Chloroplast Toolbox
(Kit # 1000000156 )

Depositing Lab:   Scott Lenaghan

The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. This kit includes a library of 115 level-0 modules (47 promoters, 36 5’UTRs, 9 promoter-5’UTR fusions, 10 3’UTRs and 13 genes of interest) along with 7 level-2 destination vectors specific for either tobacco (Nicotiana tabacum) or potato (Solanum tuberosum).

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$600 USD + shipping

Available to academics and nonprofits only.

Original Publication

MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Occhialini A, Piatek AA, Pfotenhauer AC, Frazier TP, Stewart CN Jr, Lenaghan SC. Plant Physiology. 2019 Mar; 179(3):943-957. doi: 10.1104/pp.18.01220. PubMed PMID: 30679266.

Description

The MoChlo Toolbox is a standardized library of chloroplast-specific parts to allow fast and flexible design of synthetic operons for chloroplast transformation and metabolic engineering in plants. Based on Golden-Gate cloning and compatible with previous plant MoClo systems, the MoChlo Toolbox includes a library of 115 level-0 modules (47 promoters, 36 5’UTRs, 9 promoter-5’UTR fusions, 10 3’UTRs and 13 genes of interest) along with 7 level-2 destination vectors specific for either tobacco (Nicotiana tabacum) or potato (Solanum tuberosum).

Modular Cloning Chloroplast Toolbox overview

Schematic representation of the Modular Cloning Chloroplast Toolbox (MoChlo) for assembly of chloroplast-specific transformation vectors. (A) Standardized chloroplast-specific Level-0 modules; (B) Level-1 assembly of individual expression cassettes (from 1 to 7); (C) Level-2 assembly of a synthetic operon integrated into a chloroplast transformation vector.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The MoChlo: Modular Cloning Chloroplast Toolbox was a gift from Scott Lenaghan (Addgene kit #1000000156).”

For your Reference section:

MoChlo: A Versatile, Modular Cloning Toolbox for Chloroplast Biotechnology. Occhialini A, Piatek AA, Pfotenhauer AC, Frazier TP, Stewart CN Jr, Lenaghan SC. Plant Physiology. 2019 Mar; 179(3):943-957. doi: 10.1104/pp.18.01220. PubMed PMID: 30679266.

MoChlo: Modular Cloning Chloroplast Toolbox - #1000000156

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Kanamycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pMK-PaccD (-82 to +23)
Kanamycin
Plate 1 / A / 2 pMK-PaccD (functional)
Kanamycin
Plate 1 / A / 3 pMK-PatpB (NEP functional PEP disabled)
Kanamycin
Plate 1 / A / 4 pMK-PatpB (NEP)
Kanamycin
Plate 1 / A / 5 pMK-PatpB (PEP and NEP functional)
Kanamycin
Plate 1 / A / 6 pMK-PatpB (PEP functional NEP disabled)
Kanamycin
Plate 1 / A / 7 pMK-PatpB (PEP)
Kanamycin
Plate 1 / A / 8 pMK-PatpH
Kanamycin
Plate 1 / A / 9 pMK-PatpI (NEP)
Kanamycin
Plate 1 / A / 10 pMK-PatpI (PEP Hypothetical)
Kanamycin
Plate 1 / A / 11 pMK-PclpP
Kanamycin
Plate 1 / A / 12 pMK-PclpP (PEP)
Kanamycin
Plate 1 / B / 1 pMK-IEE
Kanamycin
Plate 1 / B / 2 pMK-Prrn (-64 to +17)
Kanamycin
Plate 1 / B / 3 pMK-mPrrn (5-fold decrease transcription)
Kanamycin
Plate 1 / B / 4 pMK-PpsbA
Kanamycin
Plate 1 / B / 5 pMK-PpsbA (-42 to +9)
Kanamycin
Plate 1 / B / 6 pMK-PpsbB
Kanamycin
Plate 1 / B / 7 pMK-PpsbD-leader
Kanamycin
Plate 1 / B / 8 pMK-PpsbD-PGTbx
Kanamycin
Plate 1 / B / 9 pMK-PpsbD
Kanamycin
Plate 1 / B / 10 pMK-PrbcL
Kanamycin
Plate 1 / B / 11 pMK-PrbcL (core -35 to +9)
Kanamycin
Plate 1 / B / 12 pMK-PrbcL (-110 to +9)
Kanamycin
Plate 1 / C / 1 pMK-PrpoB
Kanamycin
Plate 1 / C / 2 pMK-Prrn16
Kanamycin
Plate 1 / C / 3 pMK-Prrn (N. tabacum)
Kanamycin
Plate 1 / C / 4 pMK-Prrn (N. tabacum) APrrn1
Kanamycin
Plate 1 / C / 5 pMK-Prrn (N. tabacum) APrrn2
Kanamycin
Plate 1 / C / 6 pMK-Prrn (N. tabacum) APrrn3
Kanamycin
Plate 1 / C / 7 pMK-Prrn (N. tabacum) APrrn4
Kanamycin
Plate 1 / C / 8 pMK-Prrn (N. tabacum) APrrn5
Kanamycin
Plate 1 / C / 9 pMK-Prrn (N. tabacum) APrrn6
Kanamycin
Plate 1 / C / 10 pMK-Prrn (N. tabacum) APrrn7
Kanamycin
Plate 1 / C / 11 pMK-Prrn (N. tabacum) APrrn8
Kanamycin
Plate 1 / C / 12 pMK-Prrn (N. tabacum) APrrn9
Kanamycin
Plate 1 / D / 1 pMK-Prrn (N. tabacum) APrrn10
Kanamycin
Plate 1 / D / 2 pMK-Prrn (N. tabacum) BPrrn12
Kanamycin
Plate 1 / D / 3 pMK-Prrn (N. tabacum) BPrrn13
Kanamycin
Plate 1 / D / 4 pMK-Prrn (N. tabacum) BPrrn14
Kanamycin
Plate 1 / D / 5 pMK-Prrn (N. tabacum) BPrrn15
Kanamycin
Plate 1 / D / 6 pMK-Prrn (N. tabacum) BPrrn16
Kanamycin
Plate 1 / D / 7 pMK-Prrn (N. tabacum) BPrrn17
Kanamycin
Plate 1 / D / 8 pMK-Prrn (N. tabacum) BPrrn18
Kanamycin
Plate 1 / D / 9 pMK-Prrn (N. tabacum) BPrrn19
Kanamycin
Plate 1 / D / 10 pMK-Prrn (N. tabacum) BPrrn20
Kanamycin
Plate 1 / D / 11 pMK-Prrn (N. tabacum) BPrrn21
Kanamycin
Plate 1 / D / 12 pMK-Zm PclpP::5'UTR
Kanamycin
Plate 1 / E / 1 pMK-Zm PclpP::Gg10 leader
Kanamycin
Plate 1 / E / 2 pMK-PaccD::5'UTR
Kanamycin
Plate 1 / E / 3 pMK-mPrrn::RBS (3.9%)
Kanamycin
Plate 1 / E / 4 pOK-mPrrn::RBS (20%)
Kanamycin
Plate 1 / E / 5 pMK-mPrrn::RBS (46%)
Kanamycin
Plate 1 / E / 6 pMK-mPrrn::RBS (60%)
Kanamycin
Plate 1 / E / 7 pMK-mPrrn::RBS (61%)
Kanamycin
Plate 1 / E / 8 pMK-Prrn::RBS
Kanamycin
Plate 1 / E / 9 pMK-atpB 5'UTR
Kanamycin
Plate 1 / E / 10 pMK-atpB 5'UTR +10 (functional)
Kanamycin
Plate 1 / E / 11 pMK-atpB 5'UTR +10 processed -90 (functional)
Kanamycin
Plate 1 / E / 12 pMK-atpB 5'UTR TCR processed -90 (functional)
Kanamycin
Plate 1 / F / 1 pMK-atpH 5'UTR
Kanamycin
Plate 1 / F / 2 pMK-atpH 5'UTR processed -45
Kanamycin
Plate 1 / F / 3 pMK-atpI 5'UTR
Kanamycin
Plate 1 / F / 4 pMK-psbN 5'UTR
Kanamycin
Plate 1 / F / 5 pMK-psbN 5'UTR processed -39
Kanamycin
Plate 1 / F / 6 pMK-psbB 5'UTR
Kanamycin
Plate 1 / F / 7 pMK-psbB 5'UTR processed -54
Kanamycin
Plate 1 / F / 8 pMK-ndhD 5'UTR
Kanamycin
Plate 1 / F / 9 pMK-rps2 5'UTR
Kanamycin
Plate 1 / F / 10 pMK-rps2 5'UTR mAAGA
Kanamycin
Plate 1 / F / 11 pMK-rps2 5'UTR mCCUC
Kanamycin
Plate 1 / F / 12 pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)
Kanamycin
Plate 1 / G / 1 pMK-rps2 5'UTR mCCUC (-20 to -10 deletion)
Kanamycin
Plate 1 / G / 2 pMK-psbH 5'UTR + start codon + 1AA
Kanamycin
Plate 1 / G / 3 pMK-psbA 5'UTR (SNM)
Kanamycin
Plate 1 / G / 4 pMK-psbA 5'UTR (SEM)
Kanamycin
Plate 1 / G / 5 pMK-psbA 5'UTR (delta SL)
Kanamycin
Plate 1 / G / 6 pMK-psbA 5'UTR (delta 5'SL)
Kanamycin
Plate 1 / G / 7 pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)
Kanamycin
Plate 1 / G / 8 pMK-psbA-rbcL chimeric 5'UTR (R'SLWT)
Kanamycin
Plate 1 / G / 9 pMK-rps14 5'UTR
Kanamycin
Plate 1 / G / 10 pMK-rbcL 5'UTR (full)
Kanamycin
Plate 1 / G / 11 pMK-rbcL 5'UTR (processed)
Kanamycin
Plate 1 / G / 12 pMK-rbcL 5'UTR + 14 AA
Kanamycin
Plate 1 / H / 1 pMK-T7G10 leader
Kanamycin
Plate 1 / H / 2 pMK-rrn16 5'UTR (native)
Kanamycin
Plate 1 / H / 3 pMK-rrn16 5'UTR (+37)
Kanamycin
Plate 1 / H / 4 pMK-psbA 5'UTR
Kanamycin
Plate 1 / H / 5 pMK-Synthetic RBS
Kanamycin
Plate 1 / H / 6 pMK-psbC 5'UTR
Kanamycin
Plate 1 / H / 7 pMK-accD 5'UTR
Kanamycin
Plate 1 / H / 8 pMK-clpP 5'UTR
Kanamycin
Plate 1 / H / 9 pMK-mEmerald
Kanamycin
Plate 1 / H / 10 pMK-pporRFP
Kanamycin
Plate 1 / H / 11 pMK-aadA
Kanamycin
Plate 1 / H / 12 pMK-aphA6
Kanamycin
Plate 2 / A / 1 pMK-nptII
Kanamycin
Plate 2 / A / 2 pMK-Myc aadA-GFP
Kanamycin
Plate 2 / A / 3 pMK-FLARE16-S aadA-GFP
Kanamycin
Plate 2 / A / 4 pMK-Lux-C codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 5 pMK-Lux-D codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 6 pMK-Lux-A codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 7 pMK-Lux-B codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 8 pMK-Lux-E codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 9 pMK-Lux-G codon opt. Nt chloroplasts
Kanamycin
Plate 2 / A / 10 pMK-psbA 3'UTR
Kanamycin
Plate 2 / A / 11 pMK-rbcL 3'UTR
Kanamycin
Plate 2 / A / 12 pMK-petD 3'UTR
Kanamycin
Plate 2 / B / 1 pMK-rpoA 3'UTR
Kanamycin
Plate 2 / B / 2 pMK-E.coli rrnB 3'UTR
Kanamycin
Plate 2 / B / 3 pMK-psaC 3'UTR
Kanamycin
Plate 2 / B / 4 pMK-TMV 3'UTR
Kanamycin
Plate 2 / B / 5 pMK-TYMV 3'UTR
Kanamycin
Plate 2 / B / 6 pMK-BMV 3'UTR
Kanamycin
Plate 2 / B / 7 pMK-ALMV 3'UTR
Kanamycin
Plate 2 / B / 8 pMK-N. tabacum trnI/trnA
Kanamycin
Plate 2 / B / 9 pMK-N. tabacum trnG/trnfM
Kanamycin
Plate 2 / B / 10 pMK-N. tabacum trnV/3'rps12
Kanamycin
Plate 2 / B / 11 pMK-N. tabacum atpB/accD
Kanamycin
Plate 2 / B / 12 pMK-S. tuberosum trnI/trnA
Kanamycin
Plate 2 / C / 1 pMK-S. tuberosum trnG/trnfM
Kanamycin
Plate 2 / C / 2 pMK-S. tuberosum trnV/3'rps12
Kanamycin
Data calculated @ 2019-11-15

Kit Plate Map - #1000000156

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