Script to identify IDs from sequencing reads available in GitHub:
Protocol for high-throughput conjugation from E. coli ST18 into rhizobial strains in 96-well microtiter plates available in the Supplemental Documents of the associated publication
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Plasmid-ID Kit was a gift from Philip Poole (Addgene kit #1000000168).”
For your Reference section:
Optimizing Rhizobium- legume symbioses by simultaneous measurement of rhizobial competitiveness and N 2 fixation in nodules Mendoza-Suárez MA, Geddes BA, Sánchez-Cañizares C, Ramírez-González RH, Kirchhelle C, Jorrrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. PubMed PMID: 32317381