| Gene/insert name: | | prostate-specific membrane antigen | |
| Alternative names: | | PSMA | |
| Insert size (bp): | | 2200 | |
| GenBank/Entrez ID of insert: | | AAA60209 | |
| Gene/insert aliases: | | FOLH1, PSM, FGCP, FOLH, GCP2, PSMA, mGCP, GCPII, NAALAD1, NAALAdase | |
| Species of gene(s): | | H. sapiens (human)
| |
| Fusion proteins or tags: | | His | |
| Terminal: | | N terminal on insert | |
| Vector backbone: | | pAcGP67A
(Search Vector Database) | |
| Backbone manufacturer: | | BD Biosciences | |
| Type of vector: | | Insect expression | |
| Backbone size (bp): | | 9761 | |
| Cloning site 5': | | Bam HI | |
| Site destroyed during cloning: | | No | |
| Cloning site 3': | | Not I | |
| Site destroyed during cloning: | | No | |
| 5' Sequencing primer: | | 5' AAT TTAA AAT gAT AAC CAT CTC gC 3'' (List of Sequencing Primers) | |
| 3' Sequencing primer: | | 5' gAA gCg ggT CCA AgT TTC CCT g 3' | |
| Bacteria resistance: | | Ampicillin | |
| High or low copy: | | Unknown | |
| Grow in standard E. coli @ 37C: | | Yes | |
| If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: | | Joseph Neale and Tomas Bzdega (Georgetown University, Washington, DC) | |
| Sequence: | | View sequence |
| Plasmid Provided In: | | DH5a |
| Principal Investigator: | | Pamela J. Bjorkman |
| Terms and Licenses: | | MTA |
Article: Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase. Davis MI et al. (Proc Natl Acad Sci U S A. 2005 Apr 26. 102(17):5981-6.
Pubmed)
Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.
Also, please include the text "Addgene plasmid 11524" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.
