Addgene - pAcUW51-CgE Plasmid Data

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Plasmid 13762: pAcUW51-CgE

Gene/insert name:		HSV-1 glycoprotein E
Alternative names:		HSV-1 gE
Insert size (bp):		552
Species of gene(s):		Herpes simplex virus type I KOS strain
Relevant mutations/deletions:		C-terminal region of the HSV-1 gE (KOS strain ectodomain) was cloned downstream of the HSV-1 leader peptide by modifying Thr22 to Ala to create an AscI site to fuse the DNA encoding residues 213-390 in frame.
Fusion proteins or tags:		His
Terminal:		C terminal on insert
Vector backbone:		pAcUW51 (Search Vector Database)
Backbone manufacturer:		BD Biosciences
Type of vector:		Insect expression
Backbone size (bp):		5863
Cloning site 5':		AscI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		CGGACCTTTAATTCAACCCAAC (List of Sequencing Primers)
3' Sequencing primer:		TTGACACCAGACCAACTGGT
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Pamela J. Bjorkman
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: Crystal structure of the HSV-1 Fc receptor bound to Fc reveals a mechanism for antibody bipolar bridging. Sprague ER et al. (PLoS Biol. 2006 Jun . 4(6):e148. Pubmed)

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