Plasmid Reference Index
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Molecular Cloning Guide
- Overview Pages - Designing your Cloning Strategy
- Plasmid Cloning by Restriction Enzyme Digest
- Plasmid Cloning by PCR
- Plasmid Modification by Annealed Oligo Cloning
- Plasmid Cloning by PCR
- Specific Protocols
- Making LB Agar Plates
- Streaking and Isolating Bacteria on an LB Agar Plate
- Inoculating a Liquid Bacterial Culture
- Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids
- Recovering Plasmid DNA from Bacterial Culture
- DNA Quantification
- Restriction Digest of Plasmid DNA
- Agarose Gel Electrophoresis
- Purifying DNA from an Agarose Gel
- DNA Ligation
- Bacterial Transformation
- Diagnostic Restriction Digest
- Streaking and Isolating Bacteria on an LB Agar Plate
Protocols for Addgene Plasmids
- Overview Page
- Specific Protocols
- Streaking a Plate from an Addgene Stab Culture
- Inoculating an Overnight Liquid Culture of your Addgene Plasmid
- Creating a Glycerol Stock of your Addgene Plasmid
- Isolating your Addgene Plasmid DNA from Bacteria
- Diagnostic Restriction Digest of your Addgene Plasmid
- Sequence Analysis of your Addgene Plasmid
- Inoculating an Overnight Liquid Culture of your Addgene Plasmid
Molecular Biology Tools and References
- Tools
- Reference Materials
Choosing a Plasmid to Clone Into
- Guides for Addgene Plasmids