Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Teichmann Retroviral Mouse Genome-wide CRISPR Knockout Library
(Pooled Library #104861)

  • Purpose

    This retroviral CRISPR library targets 18,424 mouse genes using the gRNAs designed by the Kosuke Yusa Lab. The use of retrovirus enables this library to be used for cell lines that are poorly infected by lentivirus.

  • Vector Backbone

    pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 104861 gRNA pooled library 1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with other plasmid(s) or cell lines expressing mouse Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    18,424
  • gRNAs
    90,230
  • Virus Type
    Retrovirus

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼50µL
  • Concentration
    20ng/µL

Depositor Comments

The depositing lab recommends 3-5 electroporations for amplification.

NOTE: Retroviral vectors can recombine during library amplification, resulting in an ~2kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain retroviral packaging sequences or mammalian selection markers. If this 2kb band forms the predominant product after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Retroviral Mouse Genome-wide CRISPR Knockout Library was a gift from Sarah Teichmann (Addgene #104861)
  • For your References section:

    Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation. Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, Teichmann SA. Cell. 2019 Jan 7. pii: S0092-8674(18)31569-1. doi: 10.1016/j.cell.2018.11.044. PubMed 30639098.