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Weissman Lab Target Site Library (PCT48)
(Pooled Library #170392)

  • Purpose

    This lentiviral library includes a constitutively expressed GFP cassette with a lineage tracing "Target Site". The 3' UTR of GFP contains three Cas9-targetable spacer sequences and a random 14-bp barcode at high sequence diversity.

    The three cut-sites in the Target Site are complementary to the three sgRNAs expressed from the triple-sgRNA-BFP-Puromycin construct, Plasmid 170391: PCT62.

  • Vector Backbone

    PCT5

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 170392 PCT48 1 $600 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Target Site Library (PCT48) was a gift from Jonathan Weissman (Addgene #170392)
  • For your References section:

    Inference of single-cell phylogenies from lineage tracing data using Cassiopeia. Jones MG, Khodaverdian A, Quinn JJ, Chan MM, Hussmann JA, Wang R, Xu C, Weissman JS, Yosef N. Genome Biol. 2020 Apr 14;21(1):92. doi: 10.1186/s13059-020-02000-8. PubMed 32290857
    Single-cell lineages reveal the rates, routes, and drivers of metastasis in cancer xenografts. Quinn JJ, Jones MG, Okimoto RA, Nanjo S, Chan MM, Yosef N, Bivona TG, Weissman JS. Science. 2021 Feb 26;371(6532). pii: science.abc1944. doi: 10.1126/science.abc1944. Epub 2021 Jan 21. PubMed 33479121