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Addgene Guides & Resources

Kits

dRMCE Plasmids
Hamdoun lab FP fusion plasmids
Integrated Genomics Plasmids
SapTrap Kit
pCri System
cpFRET
biGBac Kit
BIOFAB
FREQ-Seq Kit
GreenGate
ZFC Modular Assembly Kit v1.0B
Wnt Open Source
FX cloning Kit
Cancer Pathways ORFs Kit
hCRISPRa-v2, genome-wide, 10 sgRNAs/gene
pDGE Dicot Genome Editing Kit
Musunuru-Cowan TALEN Kit
RAS mutant clone collection
CCSB-Broad Human Kinase ORF Collection
TALE Toolbox
ZFC Modular Assembly Kit v1.0T
Toronto KnockOut Library (TKOv1)
MXS Chaining Kit
hCRISPRi-v2, genome-wide, 10 sgRNAs/gene
GoldenBraid 2.0 Kit
Ekker Lab TALEN kit
Synuclein VHH Immune Library
Yamamoto Lab TALEN Accessory Pack
MoClo Plant Parts Kit
MoClo Yeast Toolkit
LIC TAL Effector Assembly Kit
Human SAM library in lentiSAMv2 backbone
Endy/Shearwin pOSIP plasmid set
EcoFlex MoClo Kit
Yeast Prototrophy Kit
EasyClone 2.0 Yeast Toolkit
Golden GATEway Cloning Kit
CIDAR MoClo Parts Kit
Winslow Lab Retroviral Barcoding Library
mCRISPRi-v2, genome-wide, 10 sgRNAs/gene
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
MoClo Tool Kit
PRESTO-Tango Kit
PUF Assembly Kit
RAS Pathway 2.0 Clone Collection
Wolfe-Lawson ZFN modular assembly kit
FusX TALEN assembly system
Multiplex CRISPR/Cas9 Assembly System Kit
Golden Gate TALEN and TAL effector kit 2.0
Joung Lab REAL Assembly TALEN Kit
Platinum Gate TALEN Kit
Myristoylated Kinase Library
CRISPRainbow Kit for Multiplex Labeling
C. elegans miniMos transgenesis kit
CRISPaint Gene Tagging Kit
MultiSite Gateway plasmid toolKit
mCRISPRa-v2, genome-wide, 10 sgRNAs/gene
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Zinc Finger Consortium Expression Vector Kit v1.0
Multiple Lentiviral Expression (MuLE) system
Alexandrov Cell-Free Protein Expression Kit (PEK)
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
S. cerevisiae Advanced Gateway Destination Vectors
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Zinc Finger Consortium OPEN Accessory Reagent Set
Rinehart & Söll Phosphoprotein Synthesis Kit
Human Kinase Domain Constructs for Automated Bacterial Expression
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Sabatini/Lander Lab Activity-Optimized Human CRISPR sgRNA Library
Human SAM library in lenti sgRNA(MS2)_puro backbone
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone
Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmids

  1. pREG(R1-R3) #BGV141 (Plasmid #48958) Empty backbone
    Purpose Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Retroviral
    Deposited by: David Dankort
  2. pMyc4ElbLuc. (Plasmid #53246)
    Purpose reporter plasmid containing 4 myc cDNA binding sites
    Insert: pMyc4ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  3. pLEG(R1-R3) #BGV149 (Plasmid #48956) Empty backbone
    Purpose Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: David Dankort
  4. pMyc3ElbLuc (Plasmid #53245)
    Purpose reporter plasmid containing 3 myc cDNA binding sites
    Insert: pMyc3ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  5. BL21∆serB (Bacterial strain #34929)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  6. MG1655 ΔendA ΔrecA (Bacterial strain #37853)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  7. HL 6777 (Bacterial strain #69781)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  8. HL 3500 (Bacterial strain #69774)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  9. pMyc2ElbLuc, (Plasmid #53244)
    Purpose reporter plasmid containing 2 myc cDNA binding sites
    Insert: pMyc2ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  10. Top10∆serB (Bacterial strain #34928)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  11. MM39 (Bacterial strain #42894)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Bryan Berger
  12. rEc2 (Bacterial strain #51946)
    Purpose oriT-kan template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
  13. HL 6776 (Bacterial strain #69780)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  14. HL 6703 (Bacterial strain #69778)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  15. HL 1951 (Bacterial strain #69773)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  16. pMyc1ElbLuc, (Plasmid #53243)
    Purpose reporter plasmid containing 1 myc cDNA binding sites
    Insert: pMyc1ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  17. CY008 (Bacterial strain #72401)
    Purpose Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Matthew Bennett
  18. MG1655 ΔendA ΔrecA (DE3) (Bacterial strain #37854)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  19. EcNR2.xseA- (Bacterial strain #40802)
    Tags: N/A
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  20. rEc 27.H.Sc (Bacterial strain #51948)
    Purpose specR template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
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