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Addgene Guides & Resources

Kits

dRMCE Plasmids
Integrated Genomics Plasmids
Hamdoun lab FP fusion plasmids
Wnt Open Source
SapTrap Kit
pCri System
FX cloning Kit
EcoFlex MoClo Kit
TALE Toolbox
cpFRET
MXS Chaining Kit
Ekker Lab TALEN kit
MoClo Tool Kit
PUF Assembly Kit
PRESTO-Tango Kit
BIOFAB
FREQ-Seq Kit
GreenGate
ZFC Modular Assembly Kit v1.0B
Cancer Pathways ORFs Kit
Musunuru-Cowan TALEN Kit
Yamamoto Lab TALEN Accessory Pack
MoClo Plant Parts Kit
LIC TAL Effector Assembly Kit
MoClo Yeast Toolkit
Endy/Shearwin pOSIP plasmid set
Golden GATEway Cloning Kit
Human SAM library in lentiSAMv2 backbone
CIDAR MoClo Parts Kit
EasyClone 2.0 Yeast Toolkit
CCSB-Broad Human Kinase ORF Collection
ZFC Modular Assembly Kit v1.0T
Toronto KnockOut Library (TKOv1)
GoldenBraid 2.0 Kit
Synuclein VHH Immune Library
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
RAS Pathway 2.0 Clone Collection
Wolfe-Lawson ZFN modular assembly kit
FusX TALEN assembly system
Multiplex CRISPR/Cas9 Assembly System Kit
Golden Gate TALEN and TAL effector kit 2.0
Joung Lab REAL Assembly TALEN Kit
Platinum Gate TALEN Kit
Myristoylated Kinase Library
C. elegans miniMos transgenesis kit
CRISPRainbow Kit for Multiplex Labeling
MultiSite Gateway plasmid toolKit
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Zinc Finger Consortium Expression Vector Kit v1.0
Multiple Lentiviral Expression (MuLE) system
Alexandrov Cell-Free Protein Expression Kit (PEK)
S. cerevisiae Advanced Gateway Destination Vectors
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Zinc Finger Consortium OPEN Accessory Reagent Set
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Rinehart & Söll Phosphoprotein Synthesis Kit
Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Human SAM library in lenti sgRNA(MS2)_puro backbone
Sabatini/Lander Lab Activity-Optimized Human CRISPR sgRNA Library
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

Plasmids

  1. pREG(R1-R3) #BGV141 (Plasmid #48958) Empty backbone
    Purpose Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Retroviral
    Deposited by: David Dankort
  2. pMyc4ElbLuc. (Plasmid #53246)
    Purpose reporter plasmid containing 4 myc cDNA binding sites
    Insert: pMyc4ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  3. pLEG(R1-R3) #BGV149 (Plasmid #48956) Empty backbone
    Purpose Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: David Dankort
  4. pMyc3ElbLuc (Plasmid #53245)
    Purpose reporter plasmid containing 3 myc cDNA binding sites
    Insert: pMyc3ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  5. BL21∆serB (Bacterial strain #34929)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  6. MG1655 ΔendA ΔrecA (Bacterial strain #37853)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  7. HL 6777 (Bacterial strain #69781)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  8. HL 3500 (Bacterial strain #69774)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  9. pMyc2ElbLuc, (Plasmid #53244)
    Purpose reporter plasmid containing 2 myc cDNA binding sites
    Insert: pMyc2ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  10. Top10∆serB (Bacterial strain #34928)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  11. rEc2 (Bacterial strain #51946)
    Purpose oriT-kan template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
  12. MM39 (Bacterial strain #42894)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Bryan Berger
  13. HL 6776 (Bacterial strain #69780)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  14. HL 6703 (Bacterial strain #69778)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  15. HL 1951 (Bacterial strain #69773)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  16. pMyc1ElbLuc, (Plasmid #53243)
    Purpose reporter plasmid containing 1 myc cDNA binding sites
    Insert: pMyc1ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  17. nuc5- (Bacterial strain #40804)
    Tags: None
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  18. HL 6825 (Bacterial strain #69784)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  19. HL444 (Bacterial strain #69772)
    Purpose MC4100 + agn43 gene deleted + Δdam
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  20. MG1655 ΔendA ΔrecA (DE3) (Bacterial strain #37854)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
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