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Addgene Guides & Resources

Kits

dRMCE Plasmids
Hamdoun lab FP fusion plasmids
Integrated Genomics Plasmids
TALE Toolbox
cpFRET
Ekker Lab TALEN kit
MXS Chaining Kit
Wnt Open Source
FX cloning Kit
pCri System
SapTrap Kit
FREQ-Seq Kit
BIOFAB
MoClo Tool Kit
PUF Assembly Kit
PRESTO-Tango Kit
GreenGate
ZFC Modular Assembly Kit v1.0T
CCSB-Broad Human Kinase ORF Collection
Toronto KnockOut Library (TKOv1)
LIC TAL Effector Assembly Kit
Yamamoto Lab TALEN Accessory Pack
Endy/Shearwin pOSIP plasmid set
MoClo Plant Parts Kit
Golden GATEway Cloning Kit
CIDAR MoClo Parts Kit
MoClo Yeast Toolkit
EasyClone 2.0 Yeast Toolkit
ZFC Modular Assembly Kit v1.0B
Musunuru-Cowan TALEN Kit
Cancer Pathways ORFs Kit
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
Wolfe-Lawson ZFN modular assembly kit
FusX TALEN assembly system
RAS Pathway 2.0 Clone Collection
Myristoylated Kinase Library
Joung Lab REAL Assembly TALEN Kit
Golden Gate TALEN and TAL effector kit 2.0
C. elegans miniMos transgenesis kit
Platinum Gate TALEN Kit
MultiSite Gateway plasmid toolKit
Multiplex CRISPR/Cas9 Assembly System Kit
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
S. cerevisiae Advanced Gateway Destination Vectors
Zinc Finger Consortium Expression Vector Kit v1.0
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Multiple Lentiviral Expression (MuLE) system
Alexandrov Cell-Free Protein Expression Kit (PEK)
Zinc Finger Consortium OPEN Accessory Reagent Set
Rinehart & Söll Phosphoprotein Synthesis Kit
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Sabatini/Lander Lab Activity-Optimized Human CRISPR sgRNA Library
Human SAM library in lenti sgRNA(MS2)_puro backbone

Plasmids

  1. pREG(R1-R3) #BGV141 (Plasmid #48958) Empty backbone
    Purpose Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Retroviral
    Deposited by: David Dankort
  2. pLEG(R1-R3) #BGV149 (Plasmid #48956) Empty backbone
    Purpose Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: David Dankort
  3. pMyc4ElbLuc. (Plasmid #53246)
    Purpose reporter plasmid containing 4 myc cDNA binding sites
    Insert: pMyc4ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  4. pMyc3ElbLuc (Plasmid #53245)
    Purpose reporter plasmid containing 3 myc cDNA binding sites
    Insert: pMyc3ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  5. BL21∆serB (Bacterial strain #34929)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  6. MG1655 ΔendA ΔrecA (Bacterial strain #37853)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  7. HL 3500 (Bacterial strain #69774)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  8. HL 6777 (Bacterial strain #69781)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  9. Top10∆serB (Bacterial strain #34928)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  10. MM39 (Bacterial strain #42894)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Bryan Berger
  11. rEc2 (Bacterial strain #51946)
    Purpose oriT-kan template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
  12. HL 1951 (Bacterial strain #69773)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  13. HL 6703 (Bacterial strain #69778)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  14. HL 6776 (Bacterial strain #69780)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  15. pMyc1ElbLuc, (Plasmid #53243)
    Purpose reporter plasmid containing 1 myc cDNA binding sites
    Insert: pMyc1ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  16. pMyc2ElbLuc, (Plasmid #53244)
    Purpose reporter plasmid containing 2 myc cDNA binding sites
    Insert: pMyc2ElbLuc Human
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  17. nuc4- (Bacterial strain #40803)
    Tags: None
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  18. CY007 (Bacterial strain #72337)
    Purpose Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Matthew Bennett
  19. HL372 (Bacterial strain #69771)
    Purpose MC4100 + agn43 gene deleted + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  20. HL 6779 (Bacterial strain #69783)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
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