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Addgene Guides & Resources

Plasmid Kits

Kits

dRMCE Plasmids
Hamdoun lab FP fusion plasmids
Integrated Genomics Plasmids
SapTrap Kit
pCri System
cpFRET
biGBac Kit
BIOFAB
FREQ-Seq Kit
GreenGate
ZFC Modular Assembly Kit v1.0B
Wnt Open Source
hCRISPRa-v2, genome-wide, 10 sgRNAs/gene
Cancer Pathways ORFs Kit
RAS mutant clone collection
Musunuru-Cowan TALEN Kit
FX cloning Kit
pDGE Dicot Genome Editing Kit
TALE Toolbox
CCSB-Broad Human Kinase ORF Collection
ZFC Modular Assembly Kit v1.0T
MXS Chaining Kit
Toronto KnockOut Library (TKOv1)
MoClo Pichia toolkit
hCRISPRi-v2, genome-wide, 10 sgRNAs/gene
Synuclein VHH Immune Library
GoldenBraid 2.0 Kit
Ekker Lab TALEN kit
MoClo Tool Kit
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
PRESTO-Tango Kit
PUF Assembly Kit
RAS Pathway 2.0 Clone Collection
Wolfe-Lawson ZFN modular assembly kit
pGCS vector kit
FusX TALEN assembly system
Yamamoto Lab TALEN Accessory Pack
MoClo Yeast Toolkit
Human SAM library in lentiSAMv2 backbone
MoClo Plant Parts Kit
LIC TAL Effector Assembly Kit
CIDAR MoClo Parts Kit
Golden GATEway Cloning Kit
Endy/Shearwin pOSIP plasmid set
EcoFlex MoClo Kit
Yeast Prototrophy Kit
EasyClone 2.0 Yeast Toolkit
mCRISPRi-v2, genome-wide, 10 sgRNAs/gene
STAR TALE Assembly Kit
Winslow Lab Retroviral Barcoding Library
Multiplex CRISPR/Cas9 Assembly System Kit
Golden Gate TALEN and TAL effector kit 2.0
Joung Lab REAL Assembly TALEN Kit
Platinum Gate TALEN Kit
Myristoylated Kinase Library
CRISPaint Gene Tagging Kit
C. elegans miniMos transgenesis kit
CRISPRainbow Kit for Multiplex Labeling
mCRISPRa-v2, genome-wide, 10 sgRNAs/gene
EasyClone-MarkerFree Vector Set
MultiSite Gateway plasmid toolKit
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Multiple Lentiviral Expression (MuLE) system
Zinc Finger Consortium Expression Vector Kit v1.0
SapTrap-SEC kit for C. elegans genome engineering
Alexandrov Cell-Free Protein Expression Kit (PEK)
Target Accelerator Lung Cancer Mutant Collection
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
Target Accelerator Pan-Cancer Mutant Collection
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Zinc Finger Consortium OPEN Accessory Reagent Set
Rinehart & Söll Phosphoprotein Synthesis Kit
Human Kinase Domain Constructs for Automated Bacterial Expression
S. cerevisiae Advanced Gateway Destination Vectors
Target Accelerator Genetic Pathway Reference Set
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Human SAM library in lenti sgRNA(MS2)_puro backbone
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone

Plasmids

  1. pREG(R1-R3) #BGV141 (Plasmid #48958) Empty backbone
    Purpose Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Retroviral
    Deposited by: David Dankort
  2. pLEG(R1-R3) #BGV149 (Plasmid #48956) Empty backbone
    Purpose Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: David Dankort
  3. pMyc4ElbLuc. (Plasmid #53246)
    Purpose reporter plasmid containing 4 myc cDNA binding sites
    Insert: pMyc4ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  4. pMyc3ElbLuc (Plasmid #53245)
    Purpose reporter plasmid containing 3 myc cDNA binding sites
    Insert: pMyc3ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  5. pMyc2ElbLuc, (Plasmid #53244)
    Purpose reporter plasmid containing 2 myc cDNA binding sites
    Insert: pMyc2ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  6. pMyc1ElbLuc, (Plasmid #53243)
    Purpose reporter plasmid containing 1 myc cDNA binding sites
    Insert: pMyc1ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  7. BL21∆serB (Bacterial strain #34929)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  8. MG1655 ΔendA ΔrecA (Bacterial strain #37853)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  9. HL 6777 (Bacterial strain #69781)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  10. HL 3500 (Bacterial strain #69774)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  11. MG1655 ΔendA ΔrecA (DE3) (Bacterial strain #37854)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  12. EcNR2.xseA- (Bacterial strain #40802)
    Tags: N/A
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  13. CY008 (Bacterial strain #72401)
    Purpose Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Matthew Bennett
  14. rEc 27.H.Sc (Bacterial strain #51948)
    Purpose specR template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
  15. HL 6778 (Bacterial strain #69782)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  16. HL 6686 (Bacterial strain #69775)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  17. MM39 (Bacterial strain #42894)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Bryan Berger
  18. Top10∆serB (Bacterial strain #34928)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  19. rEc2 (Bacterial strain #51946)
    Purpose oriT-kan template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
  20. HL 6776 (Bacterial strain #69780)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
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