Addgene

Showing: 61 - 80 of 142043 results

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AIDx-ID-202

Plasmid

#135344

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-202

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AIDx-ID-208

Plasmid

#135345

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-208

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AIDx-ID-468

Plasmid

#135346

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-468

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AIDx-ID-1058

Plasmid

#135347

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
BE3-NLS-NLS-P2A-BLAST

Plasmid

#135348

Purpose

Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

BE3-2XNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S2 1058 AIDx no UGI

Plasmid

#135349

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S2 1058 APOBEC3 no UGI

Plasmid

#135350

Purpose

Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-rAPOBEC3-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP APOBEC3 no UGI

Plasmid

#135352

Purpose

Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

rAPOBEC3-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 AIDx with UGI

Plasmid

#135354

Purpose

Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

hAIDx-nSpyCas9 aa-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S2 1058 ABEMax

Plasmid

#135356

Purpose

Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-ABEMax aa 1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 1

Plasmid

#135357

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 2

Plasmid

#135358

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

WT Tada-nSpyCas9 aa 1029-evolved Tada

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 CP ABEMax strategy 3

Plasmid

#135359

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 CP nSpyCas9 ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S2 1058 V82G ABEMax

Plasmid

#135361

Purpose

Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1058 nSpyCas9 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
miniABEMax V82G wt

Plasmid

#135362

Purpose

Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
S1 1029 V82G ABEMax Strategy 3

Plasmid

#135363

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
ABEMax nSaCas9

Plasmid

#135364

Purpose

Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
miniABEMax V82G nSaCas9

Plasmid

#135365

Purpose

Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax V82G nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge