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18,655 results

Showing: 161 - 180 of 18655 results

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pALPS tat-P2A-rev

Plasmid

#101331

Purpose

Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat and rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP RT-D185K/D186L

Plasmid

#101332

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts RT catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Tags

Expression

Mutation

reverse transcriptase D185K/D186L

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP IN-D116A

Plasmid

#101333

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Tags

Expression

Mutation

Integrase D116A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP PR-D25A

Plasmid

#101334

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Tags

Expression

Mutation

Protease D25A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS puro miR30-L1221

Plasmid

#101335

Purpose

negative control for knockdowns target site: CTTGTCGATGAGAGCGTTTGT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS puro miR30-IRF3

Plasmid

#101336

Purpose

target site ATCAGATCTACAATGAAGGGC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS_3'LTR GFP@gag start SFFV-

Plasmid

#101337

Purpose

Repaired U3 allows LTR-based transcription by the provirus with GFP as a marker for expression. WPRE was deleted.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS_3'LTR GFP@gag start SFFV tat

Plasmid

#101338

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS_3'LTR GFP@gag start SFFV rev

Plasmid

#101339

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP NoStarts

Plasmid

#101342

Purpose

All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

No methonines in Gag

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta GagPol

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP Δvif/vpr

Plasmid

#101344

Purpose

Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequence

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta vif/Vpr

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP Δvpu/env

Plasmid

#101345

Purpose

Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta vpu/Env

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP Δnef/U3

Plasmid

#101346

Purpose

Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta nef/LTR

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP 5'CMV Δtat

Plasmid

#101347

Purpose

tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta Tat

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta rev

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

Plasmid

#101349

Purpose

2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta tat delta TAR delta env GFP in Nef Tet ope…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALPS rtTA3_V14

Plasmid

#101350

Purpose

Codon optimized rtTA3 used in trans with Tet inducible HIV-1

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

rtTA3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 161 - 180 of 18655 results

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