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Addgene
Showing: 161 - 180 of 68401 results
  1. pHP138 Myc-TRF2-deltaB deltaM

    Plasmid
    #164255
    Purpose
    for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid
    Depositor
    Insert
    TRF2
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    deletion of amino acids-86 and 496-542
    Promoter
    Tetracycline inducible promotor
    Availability
    Academic Institutions and Nonprofits only
  2. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Availability
    Academic Institutions and Nonprofits only
  3. Nup54 no FG-mEGFP494(0)

    Plasmid
    #164269
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP
    Depositor
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. Nup54 no FG-mEGFP494(1)

    Plasmid
    #164270
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFP
    Depositor
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. Nup54 no FG-mEGFP494(2)

    Plasmid
    #164271
    Purpose
    Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFP
    Depositor
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    deleted the FG region of Nup54
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 161 - 180 of 68401 results