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Addgene
Showing: 61 - 80 of 839 results

  1. AA286

    Plasmid
    #215948
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD47_v6; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215948-plasmid-map-sequence-id-431417

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  2. AA290

    Plasmid
    #215949
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control cell surface
    Depositor
    John Doench
    Insert
    U6_v1; sgCD55_v4; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215949-plasmid-map-sequence-id-431450

    Enlarge

  3. AA310

    Plasmid
    #215950
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control lethal
    Depositor
    John Doench
    Insert
    U6_v1; sgPLK1_v2 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215950-plasmid-map-sequence-id-431492

    Enlarge

  4. AA363

    Plasmid
    #215955
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Insert
    dU6:3_v1; BsmBI_v14; BsmBI_v1; trRNA_v1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215955-plasmid-map-sequence-id-431522

    Enlarge

  5. AA364

    Plasmid
    #215956
    Purpose
    Fragmid fragment: (guide cassette) epegRNA expression
    Depositor
    John Doench
    Insert
    dU6:3_v1; BsmBI_v14; BsmBI_v15; evopreQ1_v1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215956-plasmid-map-sequence-id-431521

    Enlarge

  6. AA390

    Plasmid
    #215957
    Purpose
    Fragmid fragment: (guide cassette) guide expression; positive control reporter
    Depositor
    John Doench
    Insert
    U6_v1; sgEGFP_v1 [Sp]; trRNA_v4 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215957-plasmid-map-sequence-id-431547

    Enlarge

  7. AA427

    Plasmid
    #215961
    Purpose
    Fragmid fragment: (guide cassette) enables iBar UMIs
    Depositor
    John Doench
    Insert
    U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215961-plasmid-map-sequence-id-431415

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  8. AA028

    Plasmid
    #216008
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Insert
    nCas9 (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216008-plasmid-map-sequence-id-431426

    Enlarge

  9. AA029

    Plasmid
    #216009
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Insert
    nCas9-NG (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216009-plasmid-map-sequence-id-431424

    Enlarge

  10. AA030

    Plasmid
    #216010
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Insert
    nCas9-SpG (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216010-plasmid-map-sequence-id-431432

    Enlarge

  11. AA031

    Plasmid
    #216011
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Insert
    nCas9-SpRY (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216011-plasmid-map-sequence-id-431442

    Enlarge

  12. AA060

    Plasmid
    #216017
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Insert
    nCas9 (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216017-plasmid-map-sequence-id-431396

    Enlarge

  13. AA061

    Plasmid
    #216018
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Insert
    nCas9-NG (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216018-plasmid-map-sequence-id-431502

    Enlarge

  14. AA062

    Plasmid
    #216019
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Insert
    nCas9-SpG (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216019-plasmid-map-sequence-id-431405

    Enlarge

  15. AA063

    Plasmid
    #216020
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Insert
    nCas9-SpRY (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216020-plasmid-map-sequence-id-431526

    Enlarge

  16. AA183

    Plasmid
    #216025
    Purpose
    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency
    Depositor
    John Doench
    Insert
    nCas9 (H840A)_v2.1 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216025-plasmid-map-sequence-id-431472

    Enlarge

  17. AA269

    Plasmid
    #216073
    Purpose
    Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA
    Depositor
    John Doench
    Insert
    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216073-plasmid-map-sequence-id-431466

    Enlarge

  18. AA271

    Plasmid
    #216074
    Purpose
    Fragmid fragment: (CROPseq) guide expression; UMI-compatible
    Depositor
    John Doench
    Insert
    WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216074-plasmid-map-sequence-id-431520

    Enlarge

  19. pRDA_831

    Plasmid
    #216100
    Purpose
    CRISPRa, EF1a-driven dCas9-VP64 (Cas only)
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216100-plasmid-map-sequence-id-431382

    Enlarge

  20. pRDA_662

    Plasmid
    #216101
    Purpose
    CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)
    Depositor
    John Doench
    Insert
    Cas9 [Sp]
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    216101-plasmid-map-sequence-id-431411

    Enlarge

Showing: 61 - 80 of 839 results