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Addgene
Showing: 81 - 100 of 1050 results
  1. pFR-mTq2-antiFRET-P2A-K-GECO1

    Plasmid
    #191469
    Purpose
    Compare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent protein
    Depositor
    Insert
    K-GECO1
    Use
    Tags
    His-mTurquoise2-antiFRETlinker-P2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pFR-mTq2-antiFRET-P2A-CH-GECO2.1

    Plasmid
    #191470
    Purpose
    Compare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent protein
    Depositor
    Insert
    CH-GECO2.1
    Use
    Tags
    His-mTurquoise2-antiFRETlinker-P2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pFR-mTq2-antiFRET-P2A-R-GECO1

    Plasmid
    #191471
    Purpose
    Compare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent protein
    Depositor
    Insert
    R-GECO1
    Use
    Tags
    His-mTurquoise2-antiFRETlinker-P2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pDRESS-mTq2-antiFRET-P2A2-linker_noSacI

    Plasmid
    #191472
    Purpose
    Negative control for ratiometric comparison between red fluorescent proteins and sensors
    Depositor
    Insert
    none
    Use
    Tags
    His-mTurquoise2-antiFRETlinker-P2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI

    Plasmid
    #191473
    Purpose
    Negative control for ratiometric comparison between green fluorescent proteins and sensors
    Depositor
    Insert
    none
    Use
    Tags
    His-mScarletI-antiFRETlinker-P2A
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 1050 results