...challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s recent contribution to the field is a...for ratiometric in vivo imaging. Griesbeck explains, while these Twitch sensors are always bright, they...Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded... how to get that into the cells of interest,” Griesbeck said. Because they are chemical compounds, they...don’t always end up where you want them to go. Griesbeck is motivated by a particular interest in monitoring...based calcium biosensors. Perhaps most notably, the Twitch calcium sensors, which are available at Addgene...beam splitters. In addition to imaging neurons, Griesbeck adds that this color change has a particular advantage...

...FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin...):1119-31. Rose T, Goltstein PM, Portugues R, Griesbeck O. Putting a finishing touch on GECIs. Frontiers...