...PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe with Improved...useful genetically encoded H2O2 probe is pQE-30-HyPer3 (2) which he will co-express together with a plasmid...

...Doudna Labs used targeted mutagenesis to generate hyper accurate Cas9 (HypaCas9). This nuclease showed decreased...

...Doudna Lab at Berkeley recently added their own hyper accurate Cas9 variant (HypaCas9) to the pool of ...

...Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr...

... quickly. However, many scientists operate in a hyper-competitive environment where getting hired hinges...

...mobile DNA, called a transposon, that’s a bit hyperactive and likes to jump around from one location in... at rates similar to retroviral vectors. The hyperactive version of the SB transposase, SB100X, is ~100...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has ...human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Tol2 is only active in vertebrates ...

...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...

...to be hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the cell... neuron “on switch.” Neuronal inhibition: Hyperpolarization Conversely, when the inside of the neuron ...

...constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system...eLife.43322. PMID: 30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy ... The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for the demands...

...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in...Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable...

... the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn that...Ignavibacteriae phylum genome. IgnaviCas9 is a hyperthermostable Cas9 CRISPR nuclease that is active up to ...

...Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded...and the Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ...

...autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy. An adult male with a heterozygous...applicable in many situations. In the case of hypertrophic cardiomyopathy and other genetic disorders, ...

...cation channel) light-activated channels to hyperpolarize tumor cells, which resulted in tumor regression...activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin...

.... https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The ...toolbox with their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of ...

...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could ...depolarization increases the BRET signal whereas hyperpolarization decreases the signal. The authors additionally...

...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

...binding sites, which then recruit an MS2-fused hyperactive variant of activation-induced cytidine deaminase...deaminase (AID). AID normally mediates somatic hypermutation to generate diverse antibodies; in the CRISPR-X...

... gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth...

...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...