...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse... experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David...target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its name ...manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the...

...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In...

...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of ...How would the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem...

...information and a detailed protocol on how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre...here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by ...came about here. Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an...

...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...