Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
Type
Blog Post
Published
June 2, 2015, 6:06 p.m.
...increase the utility of CRISPR/Cas9, it will be necessary to improve its multiplexing capacity. Multiplexing..., the modification of multiple genes is often necessary.
Guide RNAs (gRNAs) are commonly packaged in 400...bacteria), and only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility...