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logicgate1.png Synthetic Biology: Yeast


Browse Addgene's collection of synthetic biology plasmids for use in yeast.






Yeast Plasmids

Search the table by keyword or sort by the table headings. Click on the publication link to view all plasmids available from the article. Click on the PI link to view all plasmids available from this depositor's lab.

Plasmid Purpose Gene/Insert Vector Type PI Publication
pPS293GAL1 promoter (Saccharomyces cerevisiae)Yeast Expression Silver pPS (unpublished)
pPS311GAL1 promoter (Saccharomyces cerevisiae)Yeast Expression Silver A yeast RNA-binding protein shuttles between the nucleus and the cytoplasm. Mol Cell Biol. 1994 Dec . 14(12):8399-407.
pJRL2 PHO5pr VYFP (EB#1547)PHO5 promoter (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(UASm1)pr VYFP (EB#1549)PHO5 promoter UASm1 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)PHO5 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)PHO5 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1pr VYFP (EB#1571)GAL1 promoter (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)GAL1 promoter TATA-A1T6 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)GAL1 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)GAL1 promoter TATA-C2 (Saccharomyces cerevisiae)Yeast Expression O'Shea Control of stochasticity in eukaryotic gene expression. Science. 2004 Jun 18. 304(5678):1811-4.
p405MET25MET25 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405MET3MET3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405GAL1p405GAL1 (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405GALLp405GALL (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405GALSp405GALS (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405ADH1p405ADH1 (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405CYC1p405CYC1 (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405TEF1TEF1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p405TDH3p405TDH3 (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p404ADH1ADH1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p404CYC1CYC1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p404TEF1TEF1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p404TDH3TDH3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p406ADH1ADH1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p406CYC1CYC1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p406TEF1TEF1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p406TDH3TDH3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler lab plasmids (unpublished)
p404GAL1GAL1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p404GALLGALL promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p404MET3MET3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p404MET25MET25 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p406MET25MET25 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p406GALSp406GALS (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p406GALLGALL promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p404GALSGALS promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p406MET3MET3 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
p406GAL1GAL1 promoter + polylinker + CYC1 terminator (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression Buchler Buchler Lab plasmids (unpublished)
pCYC2Bacterial Expression, Yeast Expression, Synthetic Biology Alper Re-engineering multicloning sites for function and convenience. Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
pTEF2Bacterial Expression, Yeast Expression, Synthetic Biology Alper Re-engineering multicloning sites for function and convenience. Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
pGPD2Bacterial Expression, Yeast Expression, Synthetic Biology Alper Re-engineering multicloning sites for function and convenience. Nucleic Acids Res. 2011 Aug;39(14):e92. doi: 10.1093/nar/gkr346. Epub 2011 May 17.
pUC-UAS1B1-LeumUAS1B1-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B2-LeumUAS1B2-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B3-LeumUAS1B3-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B4-LeumUAS1B4-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B5-LeumUAS1B5-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B6-LeumUAS1B6-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B7-LeumUAS1B7-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-LeumUAS1B8-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B9-LeumUAS1B9-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B10-LeumUAS1B10-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B11-LeumUAS1B11-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B12-LeumUAS1B12-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B13-LeumUAS1B13-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B14-LeumUAS1B14-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B15-LeumUAS1B15-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-LeumUAS1B16-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B20-LeumUAS1B20-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B28-LeumUAS1B28-Leum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-LeumLeum promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-TEF(1004)UAS1B16-TEF(1004) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(1004)UAS1B8-TEF(1004) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(804)UAS1B8-TEF(804) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-TEF(504)UAS1B16-TEF(504) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(504)UAS1B8-TEF(504) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEFUAS1B8-TEF promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-TEF(272)UAS1B16-TEF(272) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(272)UAS1B8-TEF(272) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-TEF(203)UAS1B16-TEF(203) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(203)UAS1B8-TEF(203) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B16-TEF(136)UAS1B16-TEF(136) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pUC-UAS1B8-TEF(136)UAS1B8-TEF(136) promoterBacterial Expression, Synthetic Biology Alper Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.
pDN-G1GhPGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-G1TGtPGal1-D12 promoter (Saccharomyces cerevisiae), tetR::yEGFP (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-G1TtPGal1-D12 promoter (Saccharomyces cerevisiae), tetR (Synthetic)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-T1TCbtPGal1-T123 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-G1TCbtPGal1-D12 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-G1GZmbhPGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-T1GZmbhPGal1-T123 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-NG1TtPGal1 (wt) promoter (Saccharomyces cerevisiae), tetR (Synthetic)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
pDN-T2dGZmxhPTETREG promoter (Synthetic), yEGFP::ZeoR (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Mapping the environmental fitness landscape of a synthetic gene circuit. PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
pDN-T2dMFotPTETREG promoter (Synthetic), rtetR-M2::FFF (Synthetic)Yeast Expression, Synthetic Biology ; Expression regulator/reporter Balazsi Mapping the environmental fitness landscape of a synthetic gene circuit. PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
pTPGI_dCas9_VP64encodes yeast-optimized dCas9_VP64 synthetic transcription factordCas9_VP64 (codon-optimized for expression in S. cerevisiae) (Saccharomyces cerevisiae)Yeast Expression, CRISPR, Synthetic Biology Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_gRNA_handle_RPR1tgRNA expression empty vector (yeast)Yeast Expression, CRISPR, Synthetic Biology Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pCMV_dCas9_VP64encodes human-optimized dCas9_VP64 synthetic transcription factordCas9_VP64 (human-codon-optimized) (Homo sapiens)Mammalian Expression, CRISPR, Synthetic Biology Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pU6_gRNA_handle_U6tgRNA expression empty vector (mammalian cells)Mammalian Expression, CRISPR, Synthetic Biology Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pCAP01The function of this material is to facilitate the capture and expression of bacterial gene clusters.Bacterial Expression, Yeast Expression, Synthetic Biology ; transformation-associated recombination cloning Moore Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 10.1073/pnas.1319584111. Epub 2014 Jan 21.
Gal4pBS2-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS1-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS12-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoterpromoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS24-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoterpromoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASGAL-CU2-PleumContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS4-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.promoterYeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS3-PleumContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASGAL-CU1-PleumContains a promoter comprised of derivatives from native promoters galactose, cyc and leucine.promoterYeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS13-PleumContains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.promoterYeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASGAL-PleumContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS34-PleumContains a promoter of two gal binding sites coupled wiht a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASGAL-PCYCContains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
PGALContains full length native galactose promoter promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASGAL-PgalContains a promoter of activating sequence derived from native galactose promoter coupled with full length native galactose promoterpromoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
Gal4pBS134-PleumContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter. promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
UASgal-A9-PleumContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used. promoter (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology ; Yeast synthetic hybrid promoter construct Alper Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng. 2012 Nov;109(11):2884-95. doi: 10.1002/bit.24552. Epub 2012 May 17.
pCASExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastS. pyogenese Cas9 (Other), RNA pol III promoter (tRNA-Tyr) (Saccharomyces cerevisiae), hepatitis delta virus ribozyme, genomic (Other), sgRNA (Synthetic)Bacterial Expression, Yeast Expression, CRISPR, Synthetic Biology Cate Selection of chromosomal DNA libraries using a multiplex CRISPR system. Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
pC-G418-YRYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).nptII gene (Other), 2 micron origin or replication and URA3 gene for S. cerevisiae (Saccharomyces cerevisiae)Ternary vector for Agrobacterium mediated transformation of fungi Haynes Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici. Fungal Genet Biol. 2015 Jun;79:102-9. doi: 10.1016/j.fgb.2015.04.015.
pRPR1(1xTetO)_gRNA_handle_RPR1taTc inducible gRNA expression vectorYeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pAV113pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV114pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV115pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV116pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV11KpRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV113.loxPpRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV115.loxPpRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV116.loxPpRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV123pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV124pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV125pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV126pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV103pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Yeast Expression, Worm Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV104pRS404 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV105pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV106pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F3BioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.F6.loxPpCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K6.loxPpCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K5.loxPpCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO3.loxPpCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO5.loxPpCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.KHpCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.KNpCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K5pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.HO6pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10.K4pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.Bacterial Expression, Yeast Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAV10pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.Bacterial Expression, Synthetic Biology Boeke Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae Transcription Units. ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub 2015 Mar 23.
pAG414GPD-dCas9-VPRpAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectordCas9-VPR (Saccharomyces cerevisiae)Yeast Expression, CRISPR, Synthetic Biology Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
pTPGI_dCas9encodes yeast-optimized dCas9 synthetic transcription factorYeast-optimized dCas9 (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_a1gRNA_RPR1tencodes a1 gRNAa1 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c1gRNA_RPR1tencodes c1 gRNAc1 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c2gRNA_RPR1tencodes c2 gRNAc2 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c3gRNA_RPR1tencodes c3 gRNAc3 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c4gRNA_RPR1tencodes c4 gRNAc4 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c5gRNA_RPR1tencodes c5 gRNAc5 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c6gRNA_RPR1tencodes c6 gRNAc6 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c7gRNA_RPR1tencodes c7 gRNAc7 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_c8gRNA_RPR1tencodes c8 gRNAc8 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_g1gRNA_RPR1tencodes g1 gRNAg1 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pRPR1_g2gRNA_RPR1tencodes g2 gRNAg2 gRNA (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
pCYC1m_yeGFPencodes yeast enhanced GFP under control of minimal pCYC1 promoteryeast enhanced GFP (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
1x_a1gRNAOp_pCYC1m_yeBFP2encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFPyeast enhanced BFP2 (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
3x_a1gRNAOp_pCYC1m_yeBFP2encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPyeast enhanced BFP2 (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
6x_a1gRNAOp_pCYC1m_yeBFP2encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPyeast enhanced BFP2 (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
12x_a1gRNAOp_pCYC1m_yeBFP2encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPyeast enhanced BFP2 (Saccharomyces cerevisiae)Yeast Expression Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11.
HcKan_Preceiving vector of promoters for the construction of standard promoter parts in YeastFab workRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HcKan_Oreceiving vector of ORFs for the construction of standard ORF parts in YeastFab workRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HcKan_Treceiving vector of terminators for the construction of standard terminator parts in YeastFab workRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT2-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT3-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT4-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT5-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT6-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT7-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT8-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT9-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT10-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
POT11-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyRFP (Other)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pRS414-URRCEN plasmid as receiving vector for exogenous pathwayURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pRS424-URRhigh copy number plasmid as receiving vector for exogenous pathwayURR1-RFP-Leu2-URR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-URR1standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUsURR1 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-URR2standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUsURR2 (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-HIS3standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUsHIS3 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-LEU2standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsLEU2 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pMV-TRP1standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUsTRP1 (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-TDH3construction of TDH3 promoter in HCKan-P receiving vectorTDH3 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-TEF2construction of TEF2 promoter in HCKan-P receiving vectorTEF2 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-P-CYC1construction of CYC1 promoter in HCKan-P receiving vectorCYC1 promoter (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-T-ADH1construction of ADH1 terminator in HCKan-P receiving vectorADH1 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-T-TEF2construction of TEF2 terminator in HCKan-P receiving vectorTEF2 terminator (Saccharomyces cerevisiae)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtEsysthesis of crtE gene in the beta-carotene pathwayycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtIsysthesis of crtI gene in the beta-carotene pathwayycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
PMV-ycrtYBsysthesis of crtYB gene in the beta-carotene pathwayycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtEconstruction of ycrtE gene in HCKan-O receiving vectorycrtE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtIconstruction of ycrtI gene in HCKan-O receiving vectorycrtI (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
HCKan-O-ycrtYBconstruction of ycrtYB gene in HCKan-O receiving vectorycrtYB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioAsysthesis of vioA gene in the violacein pathwayvioA (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioBsysthesis of vioB gene in the violacein pathwayvioB (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioCsysthesis of vioC gene in the violacein pathwayvioC (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioDsysthesis of vioD gene in the violacein pathwayvioD (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pKMV-VioEsysthesis of vioE gene in the violacein pathwayvioE (Synthetic)Synthetic Biology Dai YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Nucleic Acids Res. 2015 May 8. pii: gkv464.
pDS2CEN/ARS yeast shuttle vector with DS2 selection markerDS2Synthetic Biology Sommer Flexible metabolic pathway construction using modular and divisible selection gene regulators. Metab Eng. 2015 Sep;31:189-97. doi: 10.1016/j.ymben.2015.08.004. Epub 2015 Aug 21.
pAS1NB c Rosella Ia biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.Rosella (Synthetic)Yeast Expression Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
pAS1NB m Rosella Ia biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.Rosella (Synthetic)Yeast Expression Prescott Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
pCfB2899(X-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3034(X-3 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3035(X-4 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3036(XI-1 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2903(XI-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2904(XI-3 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3037(XI-5 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3038(XII-1 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3039(XII-2 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB3040(XII-4 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB2909(XII-5 MarkerFree)EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)Yeast Expression, CRISPR, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pCfB1975 (pUC19-PTEF1-PPGK1)Example plasmid containing a bidirectional promoterpTEF1-pPGK1 (Saccharomyces cerevisiae)Bacterial Expression, Yeast Expression, Synthetic Biology Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
pNIA-CEN-FLAG-LINXa4-Bre1Control of the E3 ligase Bre1 with LINXa in yeast.LINXa4-Bre1dNLS (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pNIA-CEN-Venus-FLAG-LINXa4-Bre1Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.Venus (Saccharomyces cerevisiae)Yeast Expression, Synthetic Biology Kuhlman Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol. 2016 Jun;12(6):399-401. doi: 10.1038/nchembio.2068. Epub 2016 Apr 18.
pMOD6-pGRR-r11-r10-yeGFPpGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.yeGFP (Synthetic)Yeast Expression, CRISPR, Synthetic Biology Klavins Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates bioRxiv 041871
pMOD8A-iRGR-r11This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.RGR-r11 (Synthetic)Yeast Expression, CRISPR, Synthetic Biology Klavins Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates bioRxiv 041871
pMOD8A-RGR-r10This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.RGR-r10 (Synthetic)Yeast Expression, CRISPR, Synthetic Biology Klavins Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 NOR gates bioRxiv 041871
p1977 (pUC19-PTDH3-PTEF1)Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1Bidirectional promoters pTDH3-pTEF1 (Saccharomyces cerevisiae)Bacterial Expression Borodina EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
PP255Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP295Expresses GFP. Figure 3. Strain 1.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP296Expresses GFP. Figure 3. Strain 2.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP297Expresses GFP. Figure 3. Strain 3.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP298Expresses GFP. Figure 3. Strain 4.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP299Expresses GFP. Figure 3. Strain 6.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP300Expresses GFP. Figure 3. Strain 7.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP318Expresses GFP. Figure 3. Strain 5.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP228Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC. GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP322Expresses GFP. Figure 3. Strain 9.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP74Supplemental Figure 3. Integration Site 1.Integration Site 1Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP75Supplemental Figure 3. Integration Site 2.Integration Site 2Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP76Supplemental Figure 3. Integration Site 3.Integration Site 3Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP77Supplemental Figure 3. Integration Site 4.Integration Site 4Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP78Supplemental Figure 3. Integration Site 5.Integration Site 5Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP79Supplemental Figure 3. Integration Site 6.Integration Site 6Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP69Supplemental Figure 3. Integration Site 7.Integration Site 7Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP67Supplemental Figure 3. Integration Site 8.Integration Site 8Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP151Supplemental Figure 3. Integration Site 9.Integration Site 9Yeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP43Figure 2. Expresses BxbI.BxbI Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP44Figure 2. Expresses R4.R4 Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP45Figure 2. Expresses TP901-1.TP901-1 Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP259Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP258Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP257Promoter: GAP, minimal Promoter: mCYC. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP247Promoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP245Promoter: GAP, minimal Promoter: AOX1. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP246Promoter: GAP6, minimal Promoter: AOX1. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP277Promoter: scTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP275Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP190Promoter: ppTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP276Promoter: GAP6, minimal Promoter: GCW14. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP273Promoter: ppTEF1, minimal Promoter: GCW14. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP263Promoter: scTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP261Promoter: GAP, minimal Promoter: GAP6. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP262Promoter: GAP6, minimal Promoter: GAP6. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP254Promoter: GAP6, minimal Promoter: GAP. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP253Promoter: GAP, minimal Promoter: GAP. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP229Promoter: ppTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP244Promoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP310245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.GFP, RFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP311246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.GFP, RFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP362255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.GFP, RFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP324246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.rHGH, IFNa2bYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP326245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.rHGH, IFNa2bYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP363255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.rHGH, IFNa2bYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP165Supplemental Figure 4. Promoter GCW14. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP152Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP87Supplemental Figure 4. Promoter scTEF1. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP164Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP149Supplemental Figure 4. Promoter WT GAP. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP93Supplemental Figure 4. No Promoter.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP154Supplemental Figure 4. Promoter GAP1. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP160Supplemental Figure 4. Promoter GAP2. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP161Supplemental Figure 4. Promoter GAP3. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP155Supplemental Figure 4. Promoter GAP4. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP162Supplemental Figure 4. Promoter GAP5. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP163Supplemental Figure 4. Promoter GAP6. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
PP153Supplemental Figure 4. Promoter GAP7. Expresses GFP.GFPYeast Expression Lu Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care. Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.

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