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Limited Use Label License: EMD Millipore/Brookhaven pET

ACADEMIC AND NON-PROFIT LABORATORY ASSURANCE LETTER

The T7 expression system is based on technology developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy and is the subject of U.S. Patent No. 5,693,489, assigned to Brookhaven Science Associates, LLC (“BSA”). BSA will grant a non-exclusive license for use of this technology, including the enclosed materials, based upon the following assurances:

1. These materials are to be used for noncommercial research purposes only. A separate license is required for any commercial use, including the use of these materials for research purposes or production purposes by any commercial entity. Information about commercial licenses may be obtained from the Office of Technology Commercialization and Partnerships, Brookhaven National Laboratory, Bldg. 490-C, P.O. Box 5000, Upton, New York 11973-5000, telephone (631) 344-7134.

2. No materials that contain the cloned copy of R7 gene 1, the gene for T7 RNA polymerase, may be distributed further to third parties outside of your laboratory, unless the recipient receives a copy of this license and agrees to be bound by its terms. This limitation applies to strains BL21 (DE3), BL21 (DE3) pLysS, and BL21 (DE3)pLysE, and any derivatives of them.

EMD Millipore Limited Use Label License

U.S. Patent No. 5,693,489 has been licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. The pET vectors are commercially available from EMD Millipore Corporation and cannot be used for any commercial purpose without prior written permission from EMD Millipore.