Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
cos4/pcos4EMBL was digested with BamHI+AvaI, the 1.1 kb fragment removed and the remaining ends filled in. Synthetic NotI linkers were inserted into the EcoRI & HindIII sites. The small fragment between EcoRI & HindIII was replaced with the pUC18 polylinker. Cosmid clones contain either 3.2 or 2.7 kb of the original vector depending on which cos site was used in packaging. This does not affect the use of NotI sites in restriction mapping of the clone. Cosmid vector with a cloning capacity of 35-45 kb, 3 cos sites, and NotI sites flanking the polylinker sites for ease of restriction mapping. Restriction digests of the clone give the following sizes (kb): EcoRI--5.3; BamHI--5.3; PstI--4.30, 0.8. (ATCC staff) PATENT PENDING. Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)