Vector Database

This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene). [1] This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning. [2] This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence). [1] Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, human cell line, simian cell line, dog cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)