EMBL4 size is between 43000 and 44000 bp. left arm is 19300. right arm is 9200 bp. The stuffer is 1400 bp. Phage with inserts have the Spi- phenotype. [3] lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in the orientation of the MCS surrounding the stuffer fragment. Medium is 1592 SM buffer. Hosts: E.coli LE392, E.coli O358, E.coli O359, E.coli K802, E.coli NM538, E.coli NM539, E.coli KW251, E.coli XL-1-Blue MRA, E.coli XL-1-Blue MRA P2. Related vectors: lambda 1059, EMBL3. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)