Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: lambda gt102
Deposited by: Jinseon Lee, Sherman M. Weissman Restriction digests of the clone give the following sizes (kb): HindIII-->23.0, 9.6, 6.6; BamHI--19.4, 12.0, 6.2, 6.0, 1.5; PstI--19.4, 6.0, 3.2, 2.7, 2.1, 1.8, 1.25, 1.2, 1.1, 0.95, 0.9, 0.8, 0.6, 0.5, 0.4, 0.3, 0.2, 0.2; EcoRI--33.5, 10.5. (ATCC staff) Cloning vector permitting positive selection of inserts and useful for construction of jumping clones and libraries. Does not cross-hybridize to sequences found in common plasmid vectors.  DNA inserted into the cloning sites interrupts the imm434 cI repressor, and thus plaque formation of nonrecombinants is repressed on high-frequency lysogeny mutant strains such as C600hflA or BNN102.  The genotype appears to be more stable in the cI434 region than lambdagt10. (personal communication) May not be distributed for commercial use. (personal communication) Differs from lambdagt101 (ATCC 77363) and lambdagt103 (ATCC 77365) only in the cI434 region. (personal communication) Medium is 1592 SM buffer. Hosts: E.coli, E.coli C600, E.coli BNN102, bacteria-free lysate. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)