Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: lambda SE4
This vector uses Spi- selection for insertion of DNA into the vector and has the ability to accept 2 - 19 kb Sau3A1, BamHI, BglII, BclI, or XhoII fragments.  This vector was constructed by joining the 4.7 kb BamHI-BglII fragment of pDPT427 (low copy number replicon + spectinomycin resistance) to the left arm of lambda1059.  With a BamHI digest, the left arm is 24.1 kb, the stuffer fragment encoding the Spi+ phenotype is 16 kb, and the right arm is 8.6 kb.  This phagemid grows lytically as phage in non-immune hosts or lysogenically as a phagemid in an immune host.  Plasmid is temperature inducible to produce infectious phage particles. A phagemid vector useful for identification of genes by complementation. Medium is 1227 LB plus ampicillin.  Hosts: E.coli M5158, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)