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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: lambda SE4

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 46000
Notes:
This vector uses Spi- selection for insertion of DNA into the vector and has the ability to accept 2 - 19 kb Sau3A1, BamHI, BglII, BclI, or XhoII fragments. [1] This vector was constructed by joining the 4.7 kb BamHI-BglII fragment of pDPT427 (low copy number replicon + spectinomycin resistance) to the left arm of lambda1059. [1] With a BamHI digest, the left arm is 24.1 kb, the stuffer fragment encoding the Spi+ phenotype is 16 kb, and the right arm is 8.6 kb. [2] This phagemid grows lytically as phage in non-immune hosts or lysogenically as a phagemid in an immune host. [1] Plasmid is temperature inducible to produce infectious phage particles. A phagemid vector useful for identification of genes by complementation. Medium is 1227 LB plus ampicillin. [1] Hosts: E.coli M5158, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37387
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified