Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Kinsella, T. M., and Nolan, G. P. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum. Gene Ther., 7: 1405-1413, 1996. This plasmid contains the full length Moloney LTRs and the extended phi sequence and carries lacZ cDNA under the transcriptional control of the 5' LTR. Outside the LTRs, it contains the PGK-l promoter that drives puromycin resistance, the EBV origin of replication, and the EBNA-l gene. The last two endow the plasmid with the capacity to replicate as an episome and develop 5-20copies/mammalian cell. More information: Kinsella, T. M., and Nolan, G. P. Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum. Gene Ther., 7: 1405-1413, 1996.