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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: M13

Source/Vendor: USB
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 6407
Notes:
Sequence was compared to the sumex tape. Known differences from Suggs & Ray are: 5538a (inserted), 5558-5559ca (tc). Replicative form is duplex. Nucleotides 5725-5997 from the origin region of the M13 duplex replicative form DNA confer an M13-dependent replication mechanism upon the plasmid vector pBR322 [3]. This M13 insert permits M13 helper-dependent replication of the hybrid plasmid in polA cells which are unable to replicate the pBR322 replicon alone [3]. The segment required for high frequency transformation by oriM13 plasmids extends a maximum of 41 nucleotides beyond the HaeIII site at positions 5867-5870, but does not include most of the 'at'-rich DNA at the right hand end of the DNA [3]. Hosts: E.coli. Related vectors: M13, fd, f1. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank: M10377
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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