Digests of the clone give the following sizes (kb): EcoRI--5.4, 3.8, 2.1; HindIII--11; PstI--11. (ATCC staff) Nucleotides 2282-2557: BamHI/SalI of pBR322 (375-650 of pBR322); 2258-4773: XhoI/SalI of S. cerevisiae LEU2 from pCV13; 4774-8528: 2 micron sequences from DB248; 8529-10672: Tth111I/EcoRI of pBR32 (2218-4360 of pBR322). (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli MM294, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)