Vector Database

ATCC size is 7200 bp. The order of the major features in this plasmid is: pUC19 - EcoRI - adenovirus 2 major late promoter/tripartite leader/splice signal - NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. [1] The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Designed primarily as an enhancer trap vector. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; HindIII--3.9, 3.3; EcoRI/HindIII--3.9, 2.7, 0.6; NotI--3.6 (doublet); BamHI--7.0, 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, E.coli, monkey COS cells, vertebrate cells. Related vectors: pUC19, pNASSbeta, pCMVbeta, pSVbeta, pTKbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)